Anticorps Polyclonal de lapin anti-SEMA4C

SEMA4C Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 28402-1-AP

Synonymes

FLJ20369, KIAA1739, M SEMA F, SEMA4C, SEMACL1, SEMAF, SEMAI, Semaphorin 4C

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Galerie de données de validation

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  WB analysis using 28402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 28402-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28402-1-AP

  Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 28402-1-AP (SEMA4C antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 28402-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 28402-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28402-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28402-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28402-1-AP (SEMA4C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

28402-1-AP cible SEMA4C dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEMA4C Protéine recombinante Ag29143
• Nom complet: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
• Masse moléculaire calculée: 833 aa, 93 kDa
• Poids moléculaire observé: 93 kDa
• Numéro d’acquisition GenBank: BC109104
• Symbole du gène: SEMA4C
• Identification du gène (NCBI): 54910
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SEMA4C is a member of transmembrane semaphorin proteins which regulate axonal guidance in the developing nervous system. Furthermore, studies have demonstrated that sema4C was also implicated in biological events outside the nervous system, such as cell migration, tumor development, and terminal myogenic differentiation (PMID: 36010604). SEMA4C is highly expressed in breast, lung, cervical, and liver cancers. SEMA4C is involved in oncogenic signaling and is a potential therapeutic target for invasive breast cancer, hepatocellular carcinoma, glioma, and osteosarcoma (PMID: 33177246).

Publications

Species	Application	Title
human	WB	Mutat Res; miR-129-2-3p binds SEMA4C to regulate HCC development and inhibit the EMT; Authors - Siyuan Ma ; KD Validated
Human		Histol Histopathol; Altered expression of the Plexin-B2 system in tuberous sclerosis complex and focal cortical dysplasia IIb lesions; Authors - Lu Dai
human	WB	Front Immunol; Integrative analysis of semaphorins family genes in colorectal cancer: implications for prognosis and immunotherapy; Authors - Jiahao Zhu

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