Anticorps Polyclonal de lapin anti-SENP1

• Phare
• Validé par KD/KO

SENP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 25349-1-AP

Synonymes

SENP1, Sentrin specific protease 1, SuPr 2

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Galerie de données de validation

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  WB analysis using 25349-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25349-1-AP (SENP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25349-1-AP

  IP Result of anti-SENP1 (IP:25349-1-AP, 4ug; Detection:25349-1-AP 1:1000) with Jurkat cells lysate 3600ug.

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  IHC staining of human ovary using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25349-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25349-1-AP (SENP1 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules HepG2, cellules Jurkat, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu ovarien humain, tissu hépatique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

25349-1-AP cible SENP1 dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SENP1 Protéine recombinante Ag18028
• Nom complet: SUMO1/sentrin specific peptidase 1
• Masse moléculaire calculée: 644 aa, 73 kDa
• Poids moléculaire observé: 73-74 kDa
• Numéro d’acquisition GenBank: BC045639
• Symbole du gène: SENP1
• Identification du gène (NCBI): 29843
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.

Publications

Species	Application	Title
human	WB	Nature; Lactate regulates cell cycle by remodeling the anaphase promoting complex; Authors - Weihai Liu ; KD Validated
human	WB	Int Immunopharmacol; Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma; Authors - Jiayuan Liang
human	WB, IHC	Cancer Med; Long noncoding RNA MCM3AP-AS1 enhances cell proliferation and metastasis in colorectal cancer by regulating miR-193a-5p/SENP1.; Authors - Mingyue Zhou
human	WB	J Pharmacol Sci; Momordin Ic induces G0/1 phase arrest and apoptosis in colon cancer cells by suppressing SENP1/c-MYC signaling pathway.; Authors - Fang Xianjun ; KD Validated
	WB	J Virol; Multiple-Site SUMOylation of FMDV 3C Protease and Its Negative Role in Viral Replication; Authors - Xiangju Wu ; KD Validated
human	WB	Autophagy; A fine-tuning mechanism underlying self-control for autophagy: deSUMOylation of BECN1 by SENP3; Authors - Kejia Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (09-07-2020)

This is a great antibody for immunoblotting.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa