Anticorps Polyclonal de lapin anti-SEPHS2

SEPHS2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 14109-1-AP

Synonymes

EC:2.7.9.3, Selenide, water dikinase 2, Selenium donor protein 2, Selenophosphate synthase 2, selenophosphate synthetase 2

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Galerie de données de validation

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  WB analysis using 14109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14109-1-AP (SEPHS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14109-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14109-1-AP (SEPHS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 14109-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14109-1-AP (SEPHS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules NCI-H1299, tissu adipeux de souris, tissu hépatique de rat, tissu pancréatique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du foie humain, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below

Informations sur le produit

14109-1-AP cible SEPHS2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEPHS2 Protéine recombinante Ag5255
• Nom complet: selenophosphate synthetase 2
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47-50 kDa
• Numéro d’acquisition GenBank: BC016643
• Symbole du gène: SEPHS2
• Identification du gène (NCBI): 22928
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SEPHS2 (Selenophosphate synthetase 2), a selenophosphate synthetase, catalyzes the conversion of selenite and adenosine triphosphate (ATP) into selenophosphate. SEPHS2 is the rate-limiting enzyme that converts selenite to selenocysteine, a key substance in the synthesis of many selenium proteins such as glutathione peroxidase, thioredoxin reductase, and formate dehydrogenase (PMID: 40750759, 31695102).

Publications

Species	Application	Title
human	WB	Mol Cell; PRDX6 dictates ferroptosis sensitivity by directing cellular selenium utilization; Authors - Junya Ito
	WB	Biomaterials; H2Se-evolving bio-heterojunctions promote cutaneous regeneration in infected wounds by inhibiting excessive cellular senescence; Authors - Fan Yang
human	WB	EMBO Mol Med; Breast cancer secretes anti-ferroptotic MUFAs and depends on selenoprotein synthesis for metastasis; Authors - Tobias Ackermann
human	IHC	Front Med (Lausanne); A glycometabolic gene signature associating with immune infiltration and chemosensitivity and predicting the prognosis of patients with osteosarcoma; Authors - Fengyan Wang
mouse,human	WB	Nat Struct Mol Biol; PRDX6 augments selenium utilization to limit iron toxicity and ferroptosis; Authors - Hiroaki Fujita
human	WB	Cell Death Dis; METTL5 regulates SEPHS2-mediated selenoprotein synthesis to promote multiple myeloma survival and progression; Authors - Junyao Jiang

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