Anticorps Polyclonal de lapin anti-SEPN1

SEPN1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 55333-1-AP

Synonymes

MDRS1, RSMD1, RSS, SELENON, Selenoprotein N

---

Galerie de données de validation

• 

  WB analysis of A549 using 55333-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55333-1-AP (SEPN1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 55333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55333-1-AP (SEPN1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat skeletal muscle using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse uterus using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 55333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 55333-1-AP (SEPN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules NIH/3T3
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu de muscle squelettique de rat, tissu pulmonaire de souris, tissu utérin de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 1 publications below

Informations sur le produit

55333-1-AP cible SEPN1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: selenoprotein N, 1
• Masse moléculaire calculée: 66 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_020451
• Symbole du gène: SEPN1
• Identification du gène (NCBI): 57190
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SEPN1 (Selenoprotein N, 1) is a selenoprotein that plays a crucial role in maintaining cellular redox homeostasis and calcium homeostasis. It is encoded by the SEPN1 gene and is widely expressed in various tissues, particularly in skeletal muscle. (PMID: 32661288)

Publications

Species	Application	Title
rat	WB	Front Endocrinol (Lausanne); Changes and related factors of blood CCN1 levels in diabetic patients; Authors - Zhao-Yu Xiang
human	WB	Antioxidants (Basel); Dietary Marginal and Excess Selenium Increased Triglycerides Deposition, Induced Endoplasmic Reticulum Stress and Differentially Influenced Selenoproteins Expression in the Anterior and Middle Intestines of Yellow Catfish Pelteobagrus fulvidraco.; Authors - Dian-Guang Zhang
mouse	WB	Sci Rep; Damage to the myogenic differentiation of C2C12 cells by heat stress is associated with up-regulation of several selenoproteins.; Authors - Jiayong Tang
mouse	WB	Mar Drugs; Combination of Fish Oil and Selenium Enhances Anticancer Efficacy and Targets Multiple Signaling Pathways in Anti-VEGF Agent Treated-TNBC Tumor-Bearing Mice.; Authors - Chih-Hung Guo
mouse,human	WB	Biol Trace Elem Res; Activation of the De Novo Serine Synthesis Pathway and Disruption of Insulin Signaling Induced by Supplemental SeMet in Vitro; Authors - Shuo Zhan
mouse	WB	Front Nutr; Selenoprotein P Regulates Synaptic Zinc and Reduces Tau Phosphorylation.; Authors - Arlene C P Kiyohara

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Aditya (Verified Customer) (06-16-2025)

works well

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

{{ptg:RelatedPrimaryAntibodies}}