Anticorps Polyclonal de lapin anti-Septin 11

Septin 11 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14672-1-AP

Synonymes

SEPT11, septin 11, Septin11

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Galerie de données de validation

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  WB analysis of human brain using 14672-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 14672-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14672-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14672-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 14672-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 14672-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14672-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14672-1-AP (Septin 11 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14672-1-AP

  IP Result of anti-Septin 11 (IP:14672-1-AP, 4ug; Detection:14672-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human ovary using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14672-1-AP (Septin 11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 14672-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14672-1-AP (Septin 11 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 14672-1-AP

  Immunofluorescent analysis of Hela cells, using Septin 11 antibody 14672-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HeLa using 14672-1-AP

  Immunofluorescent analysis of Hela cells, using Septin 11 antibody 14672-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 14672-1-AP

  Immunofluorescent analysis of HepG2 cells, using Septin 11 antibody 14672-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HeLa using 14672-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Septin 11 (14672-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain, cellules HeLa, cellules HT-1080, cellules SH-SY5Y, tissu cérébral de souris, tissu de côlon de souris, tissu d'intestin grêle de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu ovarien humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

14672-1-AP cible Septin 11 dans les applications de WB, RIP, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Septin 11 Protéine recombinante Ag6342
• Nom complet: septin 11
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC063615
• Symbole du gène: SEPT11
• Identification du gène (NCBI): 55752
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	RIP	Cancer Res; CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.; Authors - Chi Hin Wong
mouse	IF	Cytoskeleton (Hoboken); Characterization of septin expression in normal and fibrotic kidneys.; Authors - Katharina Neubauer
human	IF,WB	Cell Rep; An anillin-CIN85-SEPT9 complex promotes intercellular bridge maturation required for successful cytokinesis; Authors - Thomas C Panagiotou