Anticorps Polyclonal de lapin anti-SERCA2/ATP2A2

• Phare
• Validé par KD/KO

SERCA2/ATP2A2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (1)

N° de cat : 27311-1-AP

Synonymes

ATP2A2, SERCA2, SERCA2,ATP2A2, SERCA2b, ATP2A 2

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Galerie de données de validation

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  WB analysis using 27311-1-AP

  Boiled and unboiled mouse heart lysates were subjected to SDS PAGE followed by western blot with 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27311-1-AP

  Boiled and 37°C incubated HEK-293 cells lysates were subjected to SDS PAGE followed by western blot with 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27311-1-AP (SERCA2,ATP2A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse heart tissue using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 27311-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using SERCA2,ATP2A2 antibody (27311-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris,
• Résultats positifs en IF-Fro: tissu cardiaque de souris,
• Résultats positifs en IF/ICC: cellules C2C12, tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

27311-1-AP cible SERCA2/ATP2A2 dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SERCA2/ATP2A2 Protéine recombinante Ag26177
• Nom complet: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Masse moléculaire calculée: 1042 aa, 115 kDa
• Poids moléculaire observé: 110-115 kDa
• Numéro d’acquisition GenBank: BC035588
• Symbole du gène: SERCA2
• Identification du gène (NCBI): 488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.

Publications

Species	Application	Title
mouse	WB	Life Sci; Trimethylamine N-oxide induces cardiac diastolic dysfunction by down-regulating Piezo1 in mice with heart failure with preserved ejection fraction; Authors - Qian Chen
mouse	IF	Am J Physiol Lung Cell Mol Physiol; Role of voltage-gated Ca2+ channels and Ano1 Ca2+-activated Cl- channels in M2 muscarinic receptor-dependent contractions of murine airway smooth muscle; Authors - Srijit Ghosh
mouse	WB	JCI Insight; Critical role of Znhit1 for postnatal heart function and vacuolar cardiomyopathy.; Authors - Yingchao Shi
human	WB	Nat Commun; ER calcium depletion as a key driver for impaired ER-to-mitochondria calcium transfer and mitochondrial dysfunction in Wolfram syndrome; Authors - Mailis Liiv
human	WB	Biochem Biophys Res Commun; Mitigation of ROS-triggered endoplasmic reticulum stress by upregulating Nrf2 retards diabetic nephropathy; Authors - Xiaojiao Zeng
human	WB,IHC	FASEB J; Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis; Authors - Xiang Gao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paulina (Verified Customer) (03-26-2025)

Fixation: 2% PFA for 20min. Permeabilization/antibody dilution solution: 0.05% saponin, 5% horse serum in PBS. ATP1A1 antibody diluted in saponin solution & incubated for 1 hour at room temperature, followed by 2ary anti-rabbit conjugated to AlexaFluor 488 (1:200 in saponin solution) for 1 hour. Signal shows correct localization at ER, although it is not super strong, but this could be due to low expression in this cell line.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: MDA-MB-231 cells

SERCA2/ATP2A2 Antibody Immunofluorescence validation (1:100 dilution) in MDA-MB-231 cells (Cat no:27311-1-AP)

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