Anticorps Polyclonal de lapin anti-SERPINA10

SERPINA10 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

N° de cat : 11851-1-AP

Synonymes

PZI, Serpin A10, SERPINA10, ZPI

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Galerie de données de validation

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  WB analysis of human liver using 11851-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11851-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11851-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11851-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 11851-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11851-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11851-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 11851-1-AP

  IP Result of anti-SERPINA10 (IP:11851-1-AP, 3ug; Detection:11851-1-AP 1:1000) with L02 cells lysate 2500ug.

• 

  IHC staining of human liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11851-1-AP (SERPINA10 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11851-1-AP (SERPINA10 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11851-1-AP (SERPINA10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 11851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11851-1-AP (SERPINA10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of L02 using 11851-1-AP

  Immunofluorescent analysis of L02 cells using 11851-1-AP (SERPINA10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 11851-1-AP

  Immunofluorescent analysis of HepG2 cells, using SERPINA10 antibody 11851-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules HepG2, cellules L02, RAW264.7, tissu hépatique de souris
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu hépatique humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules L02, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

11851-1-AP cible SERPINA10 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SERPINA10 Protéine recombinante Ag2420
• Nom complet: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
• Masse moléculaire calculée: 444 aa, 51 kDa
• Poids moléculaire observé: 72 kDa, 68 kDa, 50-55 kDa
• Numéro d’acquisition GenBank: BC022261
• Symbole du gène: SERPINA10
• Identification du gène (NCBI): 51156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.