Anticorps Polyclonal de lapin anti-Protein C inhibitor

Protein C inhibitor Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10673-1-AP

Synonymes

PAI3, PCI, PLANH3, PROCI, Protein C inhibitor, SERPINA5

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Galerie de données de validation

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  WB analysis of HepG2 using 10673-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10673-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10673-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10673-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10673-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10673-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10673-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10673-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A549, cellules HeLa, cellules Jurkat, cellules L02, cellules PC-3
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du sein humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 4 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10673-1-AP cible Protein C inhibitor dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protein C inhibitor Protéine recombinante Ag1078
• Nom complet: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC008915
• Symbole du gène: SERPINA5
• Identification du gène (NCBI): 5104
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.

Publications

Species	Application	Title
human	IHC	Mod Pathol; Loss of SerpinA5 protein expression is associated with advanced-stage serous ovarian tumors.; Authors - Bijsmans Ingrid T G W IT
human	IHC	J Neuropathol Exp Neurol; Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes.; Authors - Rodriguez Fausto J FJ
rat	IHC	Proteomics Clin Appl; Proteomic analysis of human articular cartilage unravels the dyscoagulation in osteoarthritis and the potential value of serpinA5 as a biomarker for osteoarthritis.; Authors - Tengjiao Zhu
human	WB,CoIP	J Cell Mol Med; SERPINA5 promotes tumour cell proliferation by modulating the PI3K/AKT/mTOR signalling pathway in gastric cancer; Authors - Meiyang Fan
rat	ELISA,IHC	Biomolecules; Improvement in Clinical Features of L-NAME-Induced Preeclampsia-like Rats through Reduced SERPINA5 Expression; Authors - Shanshui Zeng