Anticorps Polyclonal de lapin anti-SERPINA6/CBG

SERPINA6/CBG Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 12010-1-AP

Synonymes

SERPINA6, Serpin A6, CBG, Transcortin, Corticosteroid-binding globulin

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Galerie de données de validation

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  WB analysis of human plasma using 12010-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12010-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12010-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12010-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12010-1-AP (SERPINA6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12010-1-AP (SERPINA6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 12010-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12010-1-AP (SERPINA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu plasmatique humain, cellules A375, cellules HEK-293, cellules L02
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer du foie humain, tissu de cirrhose hépatique humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

12010-1-AP cible SERPINA6/CBG dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SERPINA6/CBG Protéine recombinante Ag2623
• Nom complet: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
• Masse moléculaire calculée: 405 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC058021
• Symbole du gène: SERPINA6
• Identification du gène (NCBI): 866
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINA6, also known as corticosteroid-binding globulin or CBG, belongs to the serpin family of serine protease inhibitors, and is mainly secreted by the liver. It is an alpha-globulin protein with corticosteroid-binding properties, and involved in obesity and stress sensitivity. It is also the major transport protein for glucorticoids and progestins in the blood of most vertebrates. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency).

Publications

Species	Application	Title
mouse	WB	Food Funct; Procyanidin B1 and p-coumaric acid from whole highland barley ameliorated HFD-induced impaired glucose tolerance via small intestinal barrier and hepatic glucose metabolism; Authors - Zehua Liu
human	WB	PLoS One; Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm.; Authors - Acosta-Martin Adelina E AE

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