Anticorps Polyclonal de lapin anti-SERPINB2

SERPINB2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 16035-1-AP

Synonymes

HsT1201, Monocyte Arg serpin, PAI, PAI 2, PAI2, PAI-2, PLANH2, Serpin B2, SERPIN2, SERPINB2, Urokinase inhibitor

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Galerie de données de validation

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  WB analysis using 16035-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16035-1-AP (SERPINB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 16035-1-AP

  IP result of anti-SERPINB2 (IP:16035-1-AP, 4ug; Detection:16035-1-AP 1:300) with Jurkat cells lysate 3600ug.

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  IHC staining of mouse brain using 16035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16035-1-AP (SERPINB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16035-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16035-1-AP (SERPINB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules Jurkat
• Résultats positifs en IP: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 3 publications below

Informations sur le produit

16035-1-AP cible SERPINB2 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SERPINB2 Protéine recombinante Ag8906
• Nom complet: serpin peptidase inhibitor, clade B (ovalbumin), member 2
• Masse moléculaire calculée: 415 aa, 47 kDa
• Poids moléculaire observé: 45-47 kDa
• Numéro d’acquisition GenBank: BC012609
• Symbole du gène: SERPINB2
• Identification du gène (NCBI): 5055
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Oncotarget; Exposure to airborne PM2.5 suppresses microRNA expression and deregulates target oncogenes that cause neoplastic transformation in NIH3T3 cells.; Authors - Chunling Liu
	IHC	PLoS One; Plasminogen Activator Inhibitor-2 Plays a Leading Prognostic Role among Protease Families in Non-Small Cell Lung Cancer.; Authors - Chia-Yi Su
human	WB	J Proteomics; Proteomic profiling of human decidual immune proteins during Toxoplasma gondii infection.; Authors - Dan Zhang
human	IHC,WB	Mol Cancer; circPSD3 is a promising inhibitor of uPA system to inhibit vascular invasion and metastasis in hepatocellular carcinoma; Authors - Liangliang Xu
human	WB	Carcinogenesis; Capsaicin enhances cisplatin-induced anti-metastasis of nasopharyngeal carcinoma by inhibiting EMT and ERK signaling via SERPINB2; Authors - Yafei Xu
human	WB	Aging (Albany NY); Unveiling senescence-associated secretory phenotype in epidermal aging: insights from reversibly immortalized keratinocytes; Authors - Lu-Wen Xu

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