Anticorps Polyclonal de lapin anti-Serpin B3/4

• Phare
• Validé par KD/KO

Serpin B3/4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 26558-1-AP

Synonymes

SERPINB3/4, SERPINB3, Serpin B3, SCCA-1, Protein T4-A

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Galerie de données de validation

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  WB analysis using 26558-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26558-1-AP (SERPINB3/4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 26558-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 26558-1-AP (SERPINB3/4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 26558-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 26558-1-AP (SERPINB3/4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 26558-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26558-1-AP (SERPINB3/4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 26558-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 26558-1-AP (SERPINB3/4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules L02, tissu salivaire humain
• Résultats positifs en IHC: human intrahepatic cholangiocarcinoma tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

26558-1-AP cible Serpin B3/4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Serpin B3/4 Protéine recombinante Ag24846
• Nom complet: serpin peptidase inhibitor, clade B (ovalbumin), member 3
• Masse moléculaire calculée: 390 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC005224
• Symbole du gène: SERPINB3
• Identification du gène (NCBI): 6317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Squamous cell carcinoma antigens 1 and 2 (SCCA1 and 2, SERPIN B3 and B4) are members of the ovalbumin family of serine proteinase inhibitors. SCCA1 and SCCA2 are highly homologous proteins, 91% identical at the amino acid level, and probably evolving from a common ancestor gene. The neutral form of SCCA (SCCA1, or SERPINB3) is detected in the cytoplasm of normal and some malignant squamous cells, whereas the acidic form (SCCA2, or SERPINB4) is expressed primarily in malignant cells and is the major form found in the plasma of cancer patients. SCCA2 (SERPINB4), along with SCCA1(SERPINB3), can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation. This antibody can recognize both SerpinB3 and SerpinB4.

Publications

Species	Application	Title
human	WB	Otol Neurotol; Autophagy Is Deficient and May be Negatively Regulated by SERPINB3 in Middle Ear Cholesteatoma.; Authors - Kuen-Yao Ho
human	WB,IF	J Exp Clin Cancer Res; Super-enhancer-associated TMEM44-AS1 aggravated glioma progression by forming a positive feedback loop with Myc.; Authors - Erbao Bian ; KD Validated
human	WB,IHC,IF	Adv Sci (Weinh); HPV Enhances HNSCC Chemosensitization by Inhibiting SERPINB3 Expression to Disrupt the Fanconi Anemia Pathway; Authors - Zixian Huang ; KD Validated

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