Anticorps Polyclonal de lapin anti-Maspin

Maspin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11722-1-AP

Synonymes

maspin, Peptidase inhibitor 5, PI 5, PI5, Serpin B5, SERPINB5, SPB5

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Galerie de données de validation

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  WB analysis of HepG2 using 11722-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11722-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11722-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 11722-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11722-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 11722-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 11722-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 11722-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11722-1-AP (Maspin antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of A431 using 11722-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Maspin antibody (11722-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A431 using 11722-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.5 ug Anti-Human Maspin (11722-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Control Antibody. Cells were fixed with 90% MeOH .

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du sein humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,
• Résultats positifs en cytométrie: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11722-1-AP cible Maspin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Maspin Protéine recombinante Ag2304
• Nom complet: serpin peptidase inhibitor, clade B (ovalbumin), member 5
• Masse moléculaire calculée: 375 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC020713
• Symbole du gène: SERPINB5
• Identification du gène (NCBI): 5268
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINB5, also named as PI-5, Maspin and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.

Publications

Species	Application	Title
human	IHC	Int J Cancer; Serpin B5 is a CEA-interacting biomarker for colorectal cancer.; Authors - Baek Ji Yeon JY
human	WB	Cancer Lett; Cancer stem-like cell characteristics induced by EB virus-encoded LMP1 contribute to radioresistance in nasopharyngeal carcinoma by suppressing the p53-mediated apoptosis pathway.; Authors - Chang-Fu Yang
human	WB	Oncol Lett; iTRAQ-coupled 2D LC/MS-MS analysis of CXCR7-transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers.; Authors - Hengwei Zhang
human	IF	Cell Death Dis; FAT10 promotes chemotherapeutic resistance in pancreatic cancer by inducing epithelial-mesenchymal transition via stabilization of FOXM1 expression.; Authors - Jinfeng Zhu