Anticorps Polyclonal de lapin anti-PAI-1

PAI-1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

44

Avis (1)

N° de cat : 13801-1-AP

Synonymes

PAI, PAI 1, PAI1, PAI-1, PLANH1, Serpin E1, SERPINE1, PAI-1

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Galerie de données de validation

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  WB analysis using 13801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13801-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 13801-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13801-1-AP

  IP result of anti-PAI-1(IP:13801-1-AP, 4ug; Detection:13801-1-AP 1:2000) with HepG2 cells lysate 1360 ug.

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  IHC staining of human lung cancer using 13801-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13801-1-AP (PAI-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13801-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13801-1-AP (PAI-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 13801-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using PAI-1 antibody (13801-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HuH-7, cellules HepG2, cellules PC-12, cellules U2OS, tissu placentaire humain
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HUVEC,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 40 publications below
• IHC: See 9 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

13801-1-AP cible PAI-1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: bovin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAI-1 Protéine recombinante Ag4777
• Nom complet: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• Masse moléculaire calculée: 402 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC010860
• Symbole du gène: SERPINE1
• Identification du gène (NCBI): 5054
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and 90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).

Publications

Species	Application	Title
human	WB	Am J Respir Crit Care Med; CD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell Aging; Authors - Huachun Cui
human	WB	Cancer Discov; Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer; Authors - Nurmaa K Dashzeveg
human	CoIP,WB,IHC	Circ Res; MiR-18a inhibits BMP4 and HIF-1α Normalizing Brain Arteriovenous Malformations.; Authors - Nagore I Marín-Ramos
human	WB,IHC,IF	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB	Nat Commun; A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.; Authors - J J David Ho

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

AIKATERINI (Verified Customer) (03-15-2023)

The antibody was diluted in TBS-T 5% non fat milk.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela

PAI-1 Antibody Western Blot validation (1:1000 dilution) in Hela (Cat no:13801-1-AP)

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