Anticorps Polyclonal de lapin anti-SERPINE2

• Phare
• Validé par KD/KO

SERPINE2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 11303-1-AP

Synonymes

GDN, Glia derived nexin, Peptidase inhibitor 7, PI 7, PI7, PN 1, PN1, PNI, Protease nexin 1, Protease nexin I, Serpin E2, SERPINE2

---

Galerie de données de validation

• 

  WB analysis of C6 using 11303-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 11303-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11303-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11303-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11303-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 11303-1-AP

  IP Result of anti-SERPINE2 (IP:11303-1-AP, 3ug; Detection:11303-1-AP 1:300) with A549 cells lysate 2800ug.

• 

  IHC staining of human testis using 11303-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 11303-1-AP (SERPINE2 Antibody) at dilution of 1:50.

• 

  IHC staining of human testis using 11303-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 11303-1-AP (SERPINE2 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas cancer using 11303-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11303-1-AP (SERPINE2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of human testis using 11303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human testis using 11303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse testis using 11303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 11303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SERPINE2 antibody (11303-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules C6, cellules A549, cellules MCF-7, tissu cardiaque de rat, tissu cérébral de rat
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu testiculaire humain, cellules A549, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 3 publications below

Informations sur le produit

11303-1-AP cible SERPINE2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SERPINE2 Protéine recombinante Ag1830
• Nom complet: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44-50 kDa
• Numéro d’acquisition GenBank: BC015663
• Symbole du gène: SERPINE2
• Identification du gène (NCBI): 5270
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

Publications

Species	Application	Title
human	WB,IHC,IP	Cancer Lett; SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.; Authors - Jingjing Zhang ; KD Validated
human	WB,IHC	Respir Res; High expression level of serpin peptidase inhibitor clade E member 2 is associated with poor prognosis in lung adenocarcinoma.; Authors - Ryota Dokuni ; KD Validated
human	WB	J Immunol; Deep Coverage Tissue and Cellular Proteomics Revealed IL-1β Can Independently Induce the Secretion of TNF-Associated Proteins from Human Synoviocytes.; Authors - Shengquan Tang
human	IP	Front Cell Dev Biol; BAP31 Promotes Tumor Cell Proliferation by Stabilizing SERPINE2 in Hepatocellular Carcinoma.; Authors - Xiyang Zhang
mouse	IF	Sci Rep; PMID: 30446696; Authors - Mary Ann Stepp
human	IHC	J Cell Physiol; Transcriptional profiling of endometriosis tissues identifies genes related to organogenesis defects.; Authors - Crispi Stefania S

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Ahmad (Verified Customer) (06-18-2021)

Fix in 1x PBS, 1% formaldehyde, 2 mM MgCl2, 5 mM EGTA, 0.02% NP-40 for 1 hr works good for staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: rabbit cornea

SERPINE2 Antibody Immunofluorescence validation (1:300 dilution) in rabbit cornea (Cat no:11303-1-AP)