Anticorps Monoclonal anti-SERPINE2

SERPINE2 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

CloneNo.

1E11F12

N° de cat : 66203-1-Ig

Synonymes

GDN, Glia derived nexin, Peptidase inhibitor 7, PI 7, PI7, PN 1, PN1, PN-1, PNI, Protease nexin 1, Protease nexin I, Serpin E2, SERPINE2

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Galerie de données de validation

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  WB analysis using 66203-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of HEK-293 using 66203-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 66203-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 66203-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 66203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 66203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human testis using 66203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 66203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66203-1-Ig (SERPINE2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 66203-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SERPINE2 antibody (66203-1-Ig, Clone: 1E11F12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules hTERT-RPE1, cellules C6, cellules HEK-293, cellules MG U-87, cellules Neuro-2a, cellules U-251
• Résultats positifs en IHC: tissu placentaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

66203-1-Ig cible SERPINE2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SERPINE2 Protéine recombinante Ag1830
• Nom complet: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC015663
• Symbole du gène: SERPINE2
• Identification du gène (NCBI): 5270
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; BAP31 Promotes Tumor Cell Proliferation by Stabilizing SERPINE2 in Hepatocellular Carcinoma.; Authors - Xiyang Zhang
human	IHC	Oncol Lett; Expression pattern of human SERPINE2 in a variety of human tumors.; Authors - Ying Yang
mouse	WB,IHC	Theranostics; Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma; Authors - Lu-Ning Qin