Anticorps Recombinant de lapin anti-SERPINE2

SERPINE2 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

5O16

N° de cat : 82732-5-RR

Synonymes

PI7, PN1, Serpin E2, 5O16, GDN

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Galerie de données de validation

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  WB analysis using 82732-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82732-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 82732-5-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug SERPINE2 Recombinant antibody (82732-5-RR, Clone:5O16) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 82732-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82732-5-RR against Human SERPINE2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules MG U-87, cellules C6, cellules U-251, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82732-5-RR cible SERPINE2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SERPINE2 Protéine recombinante Ag1830
• Nom complet: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 44-50 kDa
• Numéro d’acquisition GenBank: BC015663
• Symbole du gène: SERPINE2
• Identification du gène (NCBI): 5270
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

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