Anticorps Polyclonal de lapin anti-HSP47

• Phare
• Validé par KD/KO

HSP47 Polyclonal Antibody for FC (Intra), IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

11

Avis (3)

N° de cat : 10875-1-AP

Synonymes

47 kDa heat shock protein, AsTP3, CBP1, CBP2, Colligin, gp46, HSP47, PIG14, PPROM, RA A47, Serpin H1, SERPINH1, SERPINH2

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Galerie de données de validation

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  WB analysis using 10875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 10875-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10875-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 10875-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10875-1-AP (HSP47 antibody at dilution of 1:50.

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  IF Staining of HeLa using 10875-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (10875-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10875-1-AP

  Immunofluorescent analysis of HeLa cells using 10875-1-AP (HSP47 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10875-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HSP47 (10875-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules A2780, cellules A431, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer du poumon humain, tissu de cirrhose hépatique humain, tissu de côlon humain, tissu pulmonaire de rat, tissu pulmonaire de souris, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

10875-1-AP cible HSP47 dans les applications de WB, IP, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HSP47 Protéine recombinante Ag1301
• Nom complet: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC014623
• Symbole du gène: SERPINH1
• Identification du gène (NCBI): 871
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.

Publications

Species	Application	Title
human	WB	Nat Protoc; Responsive and activable nanomedicines for remodeling the tumor microenvironment.; Authors - Yinlong Zhang ; KD Validated
human	WB,IF,IHC	Nat Commun; Reversal of pancreatic desmoplasia by re-educating stellate cells with a tumour microenvironment-activated nanosystem.; Authors - Xuexiang Han ; KD Validated
human,mouse	WB,IHC	Cancer Res; RUNX1 is a driver of renal cell carcinoma correlating with clinical outcome.; Authors - Nicholas Rooney
human	IP	mSystems; Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.; Authors - Xiaoqing Liu
mouse	WB	PLoS Genet; Cyclophilin B control of lysine post-translational modifications of skin type I collagen.; Authors - Masahiko Terajima
mouse	WB	Sci Rep; Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes; Authors - Masahiko Terajima

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Gohar (Verified Customer) (02-06-2020)

na

• Applications: Immunohistochemistry,

FH

Gohar (Verified Customer) (02-06-2020)

na

• Applications: Immunohistochemistry,

FH

Danielle (Verified Customer) (09-09-2019)

This antibody, as well as the other 5 purchased for our purposes, was sufficient and provided clear results for our experiment.

• Applications: ChIP,