Anticorps Polyclonal de lapin anti-SET/TAF-I

• Phare
• Validé par KD/KO

SET/TAF-I Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 55201-1-AP

Synonymes

2PP2A, HLA DR associated protein II, I 2PP2A, I2PP2A, IGAAD, IPP2A2, PHAPII, Protein SET, SET, SET nuclear oncogene, SET/TAF-I, TAF I, TAF IBETA, Template activating factor I

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Galerie de données de validation

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  WB analysis using 55201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55201-1-AP (SET/TAF-I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55201-1-AP (SET/TAF-I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 55201-1-AP (SET/TAF-I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 55201-1-AP (SET/TAF-I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55201-1-AP (SET antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 55201-1-AP (SET antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver cancer using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55201-1-AP (SET antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver cancer using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55201-1-AP (SET antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human gliomas using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55201-1-AP (SET Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 55201-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55201-1-AP (SET Antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IHC: tissu splénique de souris, tissu de cancer du foie humain, tissu de gliome humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below

Informations sur le produit

55201-1-AP cible SET/TAF-I dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SET nuclear oncogene
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 41 kDa, 39 kDa
• Numéro d’acquisition GenBank: NM_003011
• Symbole du gène: SET
• Identification du gène (NCBI): 6418
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SET, also named as IGAAD, I-2PP2A, TAF-I, IGAAD and PHAPII, belongs to the nucleosome assembly protein (NAP) family.SET is a multifunctional oncoprotein which is ubiquitously expressed in all kinds of cells. The SET protein participates in many cellular processes including cell cycle, cell migration, apoptosis, transcription, and DNA repair. The SET protein plays important roles in facilitating cell growth and proliferation via many pathways that promote tumorigenesis and metastasis. The SET protein shows apparent molecular masses of 41 and 39 kDa (PMID: 12759364, PMID: 7753797).

Publications

Species	Application	Title
human	WB	Cell Rep; A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression; Authors - Ling Zhang ; KO Validated
human	WB	Oncotarget; Overexpression of PP2A inhibitor SET oncoprotein is associated with tumor progression and poor prognosis in human non-small cell lung cancer.; Authors - Hao Liu ; KD Validated
human	WB	J Med Chem; Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies.; Authors - Brianna D Mackie
human	WB	Cancers (Basel); Pre-Clinical Study Evaluating Novel Protein Phosphatase 2A Activators as Therapeutics for Neuroblastoma.; Authors - Laura V Bownes
human	WB	Cell Signal; PMID: 25152373; Authors - Hao Liu
human	WB,IHC	BMC Cancer; MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer.; Authors - Hongli Pan