Anticorps Polyclonal de lapin anti-SETD7

• Phare
• Validé par KD/KO

SETD7 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 24840-1-AP

Synonymes

H3 K4 HMTase SETD7, KIAA1717, KMT7, Lysine N methyltransferase 7, SET7, SET7/9, SET9, SETD7

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Galerie de données de validation

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  WB analysis using 24840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 24840-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24840-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24840-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24840-1-AP (SETD7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 24840-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 24840-1-AP (SETD7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

24840-1-AP cible SETD7 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SETD7 Protéine recombinante Ag18219
• Nom complet: SET domain containing (lysine methyltransferase) 7
• Masse moléculaire calculée: 366 aa, 41 kDa
• Poids moléculaire observé: 48-50 kDa
• Numéro d’acquisition GenBank: BC121055
• Symbole du gène: SETD7
• Identification du gène (NCBI): 80854
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SET domain containing lysine methyltransferase 7 (SETD7, also called SET7/9) was the first lysine methyltransferase (KMT) discovered to specifically monomethylate lysine-4 of histone 3 (H3K4me1) (PMID: 26435321). SETD7 has been shown to have a very broad target specificity in vitro, including transcriptional regulators such as TAF10, p53, ER, p65, STAT3, Rb, Mypt, Tat, and Foxo3 (PMID: 25136132, 30013796).

Publications

Species	Application	Title
human	IHC,WB	EBioMedicine; Histone-lysine N-methyltransferase SETD7 is a potential serum biomarker for colorectal cancer patients.; Authors - Baojun Duan
human	WB	Oncotarget; SETD7 is a prognosis predicting factor of breast cancer and regulates redox homeostasis.; Authors - Run Huang ; KD Validated
mouse	CoIP	FASEB J; SETD7 mediates the vascular invasion in articular cartilage and chondrocytes apoptosis in osteoarthriis.; Authors - Jia Xiaoshi
human	WB	Exp Cell Res; Proteomic analysis of DZIP3 interactome and its role in proliferation and metastasis in gastric cancer cells; Authors - Rongfang Qiu
human	WB	Med Oncol; DDB1 regulates the activation-induced apoptosis of T cells via downregulating the expression of histone methyltransferase SETD7; Authors - Ruirong Wu
human	CoIP	Histol Histopathol; ZKSCAN5 activates LAPTM5 expression by recruiting SETD7 to promote metastasis in pancreatic ductal adenocarcinoma; Authors - Yong Yang