Anticorps Polyclonal de lapin anti-SETDB1

• Phare
• Validé par KD/KO

SETDB1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(93)

Avis (3)

N° de cat : 11231-1-AP

Synonymes

EC:2.1.1.366, ERG-associated protein with SET domain, ESET, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4

---

Galerie de données de validation

• 

  WB analysis of Jurkat using 11231-1-AP

  WB result of SETDB1 antibody (11231-1-AP, 1:1000) with si-Control and si-SETDB1 transfected Jurkat cells.

• 

  WB analysis using 11231-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11231-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11231-1-AP

  WB result of SETDB1 antibody (11231-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SETDB1 transfected HeLa cells.

• 

  WB analysis of human testis using 11231-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11231-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11231-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11231-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11231-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11231-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11231-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11231-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11231-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11231-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11231-1-AP (SETDB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 11231-1-AP

  IP result of anti-SETDB1 (IP:11231-1-AP, 3ug; Detection:11231-1-AP 1:1000) with MCF-7 cells lysate 2500ug.

• 

  IP experiment of MCF-7 using 11231-1-AP

  IP result of anti-SETDB1 (IP:11231-1-AP, 4ug; Detection:11231-1-AP 1:3000) with MCF-7 cells lysate 1720 ug.

• 

  IHC staining of human lung cancer using 11231-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11231-1-AP (SETDB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 11231-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11231-1-AP (SETDB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11231-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11231-1-AP (SETDB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 11231-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11231-1-AP (SETDB1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 11231-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11231-1-AP (SETDB1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 11231-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11231-1-AP (SETDB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 11231-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SETDB1 antibody (11231-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of A431 using 11231-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.4 ug SETDB1 Polyclonal antibody (11231-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  ChIP experiment of HeLa using 11231-1-AP

  Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of SETDB1 Antibody (11231-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules MDA-MB-231, cellules NIH/3T3, cellules PC-3, tissu testiculaire humain
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431,
• Résultats positifs en ChIP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• CHImmunoprécipitation (IP): CHIP : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 21 publications below
• WB: See 68 publications below
• IHC: See 10 publications below
• IF: See 12 publications below
• IP: See 9 publications below
• CoIP: See 4 publications below
• ChIP: See 24 publications below

Informations sur le produit

11231-1-AP cible SETDB1 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SETDB1 Protéine recombinante Ag1725
• Nom complet: SET domain, bifurcated 1
• Masse moléculaire calculée: 143 kDa
• Poids moléculaire observé: 170 kDa
• Numéro d’acquisition GenBank: BC009362
• Symbole du gène: SETDB1
• Identification du gène (NCBI): 9869
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SETDB1, also named as ESET, KIAA0067 and KMT1E, belongs to the histone-lysine methyltransferase family. It is a SET domain protein with histone H3-K9-specific methyltransferase activity. H3 'Lys-9' trimethylation is coordinated with DNA methylation and represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 regulates histone methylation, gene silencing, and transcriptional repression. It has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SETDB1.The calculated molecular weight of SETDB1 is 143 kDa, but the modified SETDB1 protein is about 170 kDa (PMID: 11791185).

Publications

Species	Application	Title
mouse	WB	Nature; METTL3 regulates heterochromatin in mouse embryonic stem cells.; Authors - Wenqi Xu ; KO Validated
mouse	WB,CoIP	Nature; The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.; Authors - Jiadong Liu
mouse,human	WB	Nature; Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity.; Authors - Gabriel K Griffin ; KO Validated
mouse	WB,CoIP,ChIP	Nature; KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements; Authors - Shang-Min Zhang
human	WB	Nature; Genome surveillance by HUSH-mediated silencing of intronless mobile elements.; Authors - Marta Seczynska ; KD Validated
human	WB	Science; GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.; Authors - Iva A Tchasovnikarova ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Hilda (Verified Customer) (07-29-2024)

SETDB1 antibody works very well with human and mouse cell lines for both western blot and ChIP. It requires a short incubation period and produces distinct bands, with a few nonspecific bands observed variable to the type of cells used. CHIP outcomes are still preliminary, but it has resulted in good binding at 2ug antibody used.

• Applications: Western Blot, ChIP
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 4T1 Cells

SETDB1 Antibody Western Blot,ChIP validation (1:1000 dilution) in 4T1 Cells (Cat no:11231-1-AP)

FH

Roy (Verified Customer) (06-28-2024)

Does work well to detect SETDB1 in Jurkat and HeLa cells (Overnight incubation at 4°C, 1/1000 dilution)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Jurkat/HeLa

FH

Taku (Verified Customer) (06-15-2022)

• Applications: Western Blot
• Primary Antibody Dilution: x2000
• Cell Tissue Type: T cell

{{ptg:RelatedPrimaryAntibodies}}