Anticorps Polyclonal de lapin anti-SF3A1

SF3A1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15858-1-AP

Synonymes

PRP21, PRPF21, SAP 114, SAP114, SF3A1, SF3A120, Splicing factor 3A subunit 1

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Galerie de données de validation

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  WB analysis using 15858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15858-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 15858-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15858-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15858-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15858-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15858-1-AP (SF3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15858-1-AP

  IP Result of anti-SF3A1 (IP:15858-1-AP, 4ug; Detection:15858-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 15858-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human breast cancer using 15858-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human breast cancer using 15858-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast cancer using 15858-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15858-1-AP (SF3A1 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 15858-1-AP

  Immunofluorescent analysis of HepG2 cells, using SF3A1 antibody 15858-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HEK-293, cellules HeLa, cellules K-562, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

15858-1-AP cible SF3A1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SF3A1 Protéine recombinante Ag8196
• Nom complet: splicing factor 3a, subunit 1, 120kDa
• Masse moléculaire calculée: 793 aa, 89 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC007684
• Symbole du gène: SF3A1
• Identification du gène (NCBI): 10291
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SF3A1, also known as SF3a120, is a 793 amino acid protein, which is a Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. SF3A1 also be involved in the assembly of the 'E' complex. The calculated molecular weight of SF3A1 is 88 kDa, but the modified SF3A1 is about 115-120 kDa.(PMID: 7489498 )

Publications

Species	Application	Title
human	WB	Cell Rep; SF3B1 Association with Chromatin Determines Splicing Outcomes.; Authors - Nir Kfir
mouse	WB	Front Pharmacol; LXR agonist inhibits inflammation through regulating MyD88 mRNA alternative splicing; Authors - Ni Li
human	IP	bioRxiv; Integrative identification of non-coding regulatory regions driving metastatic prostate cancer; Authors - Brian J Woo