Anticorps Polyclonal de lapin anti-SF3A3

• Phare
• Validé par KD/KO

SF3A3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 12070-1-AP

Synonymes

Spliceosome-associated protein 61, SF3a60, SAP61, SAP 61, PRP9

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Galerie de données de validation

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  WB analysis using 12070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12070-1-AP

  WB result of SF3A3 antibody (12070-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SF3A3 transfected A549 cells.

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  WB analysis using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12070-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12070-1-AP (SF3A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 12070-1-AP

  IP result of anti-SF3A3 (IP:12070-1-AP, 4ug; Detection:12070-1-AP 1:600) with Raji cells lysate 2000ug.

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  IHC staining of human lung cancer using 12070-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12070-1-AP (SF3A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12070-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12070-1-AP (SF3A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12070-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12070-1-AP (SF3A3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 12070-1-AP

  Immunofluorescent analysis of Hela cells, using SF3A3 antibody 12070-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules A549, cellules K-562, cellules NIH/3T3, cellules Raji
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below

Informations sur le produit

12070-1-AP cible SF3A3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SF3A3 Protéine recombinante Ag2708
• Nom complet: splicing factor 3a, subunit 3, 60kDa
• Masse moléculaire calculée: 501 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC011523
• Symbole du gène: SF3A3
• Identification du gène (NCBI): 10946
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	bioRxiv; Cancer-associated snaR-A noncoding RNA interacts with core splicing machinery and disrupts processing of mRNA subpopulations; Authors - Sihang Zhou
human	WB	Biochem Biophys Res Commun; Pre-ribosomal WDR74 module coordinates the early and late pre-rRNA processing stages for the NVL2-mediated regulation of 60S ribosome biogenesis; Authors - Yuya Hirooka
mouse	WB	iScience; Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice; Authors - Han Wang
human	WB	PLoS One; Exploring the level of metabolic reprogramming and the role of prognostic factor SF3A3 in hepatocellular carcinoma through integrated single-cell landscape analysis; Authors - Wanshuo Wei ; KD Validated
bovine	WB	Int J Mol Sci; Autophagy Mediates Escherichia Coli-Induced Cellular Inflammatory Injury by Regulating Calcium Mobilization, Mitochondrial Dysfunction, and Endoplasmic Reticulum Stress; Authors - Jianguo Liu

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