Anticorps Polyclonal de lapin anti-SF3B1

• Phare
• Validé par KD/KO

SF3B1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (1)

N° de cat : 27684-1-AP

Synonymes

PRP10, PRPF10, SAP 155, SAP155, SF3B1, SF3b155, Splicing factor 3B subunit 1

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Galerie de données de validation

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  WB analysis of MCF-7 using 27684-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 27684-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 27684-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27684-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27684-1-AP (SF3B1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 27684-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27684-1-AP (SF3B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 27684-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27684-1-AP (SF3B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules U2OS
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below

Informations sur le produit

27684-1-AP cible SF3B1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SF3B1 Protéine recombinante Ag26689
• Nom complet: splicing factor 3b, subunit 1, 155kDa
• Masse moléculaire calculée: 146 kDa
• Poids moléculaire observé: 146-150 kDa
• Numéro d’acquisition GenBank: NM_012433
• Symbole du gène: SF3B1
• Identification du gène (NCBI): 23451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SF3B1, also named as SAP155, is a 1304 amino acid protein, which belongs to the SF3B1 family. SF3B1 is a subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. SF3B1 may also be involved in the assembly of the 'E' complex. SF3B1 belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.

Publications

Species	Application	Title
human	WB	Gastroenterology; Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.; Authors - Yingying Cao
human	WB	EMBO Rep; A splicing factor switch controls hematopoietic lineage specification of pluripotent stem cells.; Authors - Yapu Li ; KD Validated
human	WB	Cancer Gene Ther; Pan-cancer oncogenic properties and therapeutic potential of SF3B4; Authors - Yanmei Shi
human	WB	Sci Rep; Exploring the mechanistic link between SF3B1 mutation and ring sideroblast formation in myelodysplastic syndrome; Authors - Tetsuro Ochi
human	WB	Nat Commun; Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12; Authors - Zixiang Wang
human	WB	EMBO J; A transcription-independent mechanism determines rapid periodic fluctuations of BRCA1 expression; Authors - Chen Nie

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wael (Verified Customer) (09-29-2022)

specific band between 100 and 150 kd

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hek293

SF3B1 Antibody Western Blot validation (1:2000 dilution) in Hek293 (Cat no:27684-1-AP)

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