Anticorps Polyclonal de lapin anti-ASF/SF2

• Phare
• Validé par KD/KO

ASF/SF2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (2)

N° de cat : 12929-2-AP

Synonymes

Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1

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Galerie de données de validation

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  WB analysis using 12929-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12929-2-AP (ASF/SF2 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12929-2-AP

  IP Result of anti-ASF/SF2 (IP:12929-2-AP, 3ug; Detection:12929-2-AP 1:500) with mouse brain tissue lysate 3600ug.

• 

  IHC staining of human gliomas using 12929-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12929-2-AP (ASF/SF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12929-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12929-2-AP (ASF/SF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12929-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12929-2-AP (ASF/SF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:9000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below
• RIP: See 3 publications below

Informations sur le produit

12929-2-AP cible ASF/SF2 dans les applications de WB, RIP, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ASF/SF2 Protéine recombinante Ag3626
• Nom complet: splicing factor, arginine/serine-rich 1
• Masse moléculaire calculée: 248 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC010264
• Symbole du gène: SFRS1
• Identification du gène (NCBI): 6426
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.

Publications

Species	Application	Title
mouse	WB	Mol Cell; Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.; Authors - Jing Wen
human	WB	Nat Commun; Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.; Authors - Yuhan Chen
human	WB	Nucleic Acids Res; Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.; Authors - Misbah Khan
mouse,human	IF,WB	Cell Death Differ; The long non-coding RNA PFI protects against pulmonary fibrosis by interacting with splicing regulator SRSF1.; Authors - Jian Sun
human	WB,IF	Angiogenesis; Extracellular matrix stiffness controls VEGF165 secretion and neuroblastoma angiogenesis via the YAP/RUNX2/SRSF1 axis.; Authors - Min Bao
human	CoIP	EMBO Mol Med; SRSF protein kinase 1 modulates RAN translation and suppresses CGG repeat toxicity; Authors - Indranil Malik ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katie (Verified Customer) (03-14-2022)

Works well for western blot - clean blot with one band.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 Western 1:50 IF
• Cell Tissue Type: EL4 cells / Primary Murine CD4 T cells

FH

Yan (Verified Customer) (04-28-2021)

It worked very well when I performed immunofluorescence. Signal was observed in the nucleus.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Osteosarcoma Epithelial Cells