Anticorps Polyclonal de lapin anti-SFRS2

SFRS2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

Avis (2)

N° de cat : 20371-1-AP

Synonymes

PR264, Protein PR264, SC 35, SC35, SFRS2, SFRS2A, Splicing component, 35 kDa, Splicing factor SC35, SRp30b, SRSF2

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Galerie de données de validation

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  WB analysis using 20371-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 20371-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20371-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 20371-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20371-1-AP (SFRS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20371-1-AP

  IP Result of anti-SFRS2 (IP:20371-1-AP, 4ug; Detection:20371-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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  IHC staining of human lung cancer using 20371-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20371-1-AP (SFRS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 20371-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20371-1-AP (SFRS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules A549, cellules HepG2, tissu cérébral de rat, tissu testiculaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

20371-1-AP cible SFRS2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: splicing factor, arginine/serine-rich 2
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-35 kDa
• Numéro d’acquisition GenBank: NM_003016
• Symbole du gène: SFRS2
• Identification du gène (NCBI): 6427
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SFRS2, also named as PR264 and Splicing component, 35 kDa, belongs to the splicing factor SR family. SFRS2 is necessary for the splicing of pre-mRNA. Some residue of SFRS2 is modified by phosphorylase and acetylase after translation. The molecular weight of modified SFRS2 is 35-40kDa. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. SFRS2 can bind to beta-globin mRNA and commit it to the splicing pathway. The antibody has no cross reaction to SFRS2B.

Publications

Species	Application	Title
human	WB	Nat Commun; Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.; Authors - Yuhan Chen
human	CoIP	EMBO Mol Med; SRSF protein kinase 1 modulates RAN translation and suppresses CGG repeat toxicity; Authors - Indranil Malik
human	WB	Neuroscience; The Age-dependent Elevation of miR-335-3p Leads to Reduced Cholesterol and Impaired Memory in Brain.; Authors - Obayed Raihan
mouse	WB	Cell Rep; SFRS11 Loss Leads to Aging-Associated Cognitive Decline by Modulating LRP8 and ApoE.; Authors - Obayed Raihan
mouse	IF	FASEB J; SRSF2 in Sertoli cells is essential for testicular development and spermatogenesis in mice; Authors - Xiaomei Xie
mouse	WB	Cell Rep; Aspartyl-tRNA synthetase 2 orchestrates iron-sulfur metabolism in hematopoietic stem cells via fine-tuning alternative RNA splicing; Authors - Xuan Gu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xingyuan (Verified Customer) (08-15-2023)

Very nice quality at 1:500 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: hek293t

SFRS2 Antibody Western Blot validation (1:500 dilution) in hek293t (Cat no:20371-1-AP)

FH

Xingyuan (Verified Customer) (07-17-2023)

The quality of SRSF2's antibody wasn't good. I was not able to see bands at around 37kDa (tried 3 times).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: 293T

SFRS2 Antibody Western Blot validation (1:1000 dilution) in 293T (Cat no:20371-1-AP)