Anticorps Polyclonal de lapin anti-SFTPB

• Phare
• Validé par KD/KO

SFTPB Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 13664-1-AP

Synonymes

SP-B, Pulmonary surfactant-associated proteolipid SPL(Phe), Pulmonary surfactant-associated protein B, 6 kDa protein, 18 kDa pulmonary-surfactant protein

---

Galerie de données de validation

• 

  WB analysis of A549 using 13664-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13664-1-AP (SFTPB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13664-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13664-1-AP (SFTPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 13664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13664-1-AP (SFTPB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13664-1-AP (SFTPB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 13664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13664-1-AP (SFTPB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 13664-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13664-1-AP (SFTPB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu pulmonaire humain, cellules A549
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

13664-1-AP cible SFTPB dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SFTPB Protéine recombinante Ag4576
• Nom complet: surfactant protein B
• Masse moléculaire calculée: 381 aa, 42 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC032785
• Symbole du gène: SFTPB
• Identification du gène (NCBI): 6439
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pulmonary surfactant-associated protein B(SFTPB) also named SFTP3, is a hydrophobic peptide that facilitates the formation and maintenance of a phospholipid-rich film at the alveolar air-liquid interface. SP-B is necessary for postnatal lung function. SFTPB as a risk biomarker for lung cancer. (PMID:36460583).

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Exp Hematol Oncol; Downregulation of pro-surfactant protein B contributes to the recurrence of early-stage non-small cell lung cancer by activating PGK1-mediated Akt signaling; Authors - Hao Luo ; KD Validated
mouse	WB	Int J Mol Sci; Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway.; Authors - Guangying Yang
human	WB,IHC,IF	Lab Invest; Liquid Biopsy in Lung Cancer: Nano-Flow Cytometry Detection of Non-Small Cell Lung Cancer in Blood; Authors - Andong Zhang
human	WB	Environ Toxicol; Silica nanoparticle exposure inhibits surfactant protein A and B in A549 cells through ROS-mediated JNK/c-Jun signaling pathway.; Authors - Xiaojing Yang
human	IHC	Heliyon; Therapeutic and prognostic effect of disulfidptosis-related genes in lung adenocarcinoma; Authors - Changshuan Li
mouse	IHC,IF	Int Immunopharmacol; The impact of POSTN on tumor cell behavior and the tumor microenvironment in lung adenocarcinoma; Authors - Dongfeng Sun

{{ptg:RelatedPrimaryAntibodies}}