Anticorps Polyclonal de lapin anti-Beta Sarcoglycan

Beta Sarcoglycan Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (2)

N° de cat : 11936-1-AP

Synonymes

43DAG, A3b, Beta sarcoglycan, Beta SG, LGMD2E, SGC, SGCB

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 11936-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11936-1-AP (Beta Sarcoglycan antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 11936-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11936-1-AP (Beta Sarcoglycan antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 11936-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11936-1-AP (Beta Sarcoglycan antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris,
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

11936-1-AP cible Beta Sarcoglycan dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Beta Sarcoglycan Protéine recombinante Ag2533
• Nom complet: sarcoglycan, beta (43kDa dystrophin-associated glycoprotein)
• Masse moléculaire calculée: 318 aa, 35 kDa
• Poids moléculaire observé: 45-48 kDa
• Numéro d’acquisition GenBank: BC020709
• Symbole du gène: Beta Sarcoglycan
• Identification du gène (NCBI): 6443
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SGCB is a 43-kD component of the dystrophin-glycoprotein complex.

Publications

Species	Application	Title
rat	WB	Phytomedicine; Cardioprotective effect of icariin against myocardial fibrosis and its molecular mechanism in diabetic cardiomyopathy based on network pharmacology: Role of ICA in DCM.; Authors - Liping Zhang
human	WB,IHC	Acta Histochem; Exploring the oncogenic role of RGS19 in bladder cancer progression and prognosis; Authors - Lei Yan
mouse	WB,IF	Oncotarget; Liraglutide suppresses obesity and induces brown fat-like phenotype via Soluble Guanylyl Cyclase mediated pathway in vivo and in vitro.; Authors - Endong Zhu
rat	WB	J Pharm Biomed Anal; Study on the mechanism of Xinmailong injection against chronic heart failure based on transcriptomics and proteomics; Authors - Yanni Xue
rat	WB	Pain Res Manag; Exploring the Analgesic Initiation Mechanism of Tuina in the Dorsal Root Ganglion of Minor CCI Rats via the TRPV1/TRPA1-cGMP Pathway; Authors - Zhenjie Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shushu (Verified Customer) (07-24-2025)

The antibody appears to work for the most part, as bands of the expected size are present in most of the positive samples. However, there are also numerous nonspecific bands of varying sizes. Optimization is needed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: primery muscle cells and HEK, HAP1 cells

Beta Sarcoglycan Antibody Western Blot validation (1:1000 dilution) in primery muscle cells and HEK, HAP1 cells (Cat no:11936-1-AP)

FH

Venkata (Verified Customer) (01-03-2022)

I have loaded 50 and 100ug of protein in each of the lanes and the samples are from mouse heart and skeletal muscle tissues. I don't see expected bands in all the samples and most of the bands are non specific.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse cardiac and skeletal muscle

Beta Sarcoglycan Antibody Western Blot validation (1:500 dilution) in Mouse cardiac and skeletal muscle (Cat no:11936-1-AP)

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