Anticorps Polyclonal de lapin anti-SGMS1

• Phare
• Validé par KD/KO

SGMS1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 19050-1-AP

Synonymes

EC:2.7.8.27, hmob33, Medulla oblongata-derived protein, MOB, MOB1

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Galerie de données de validation

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  WB analysis of human heart using 19050-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19050-1-AP (SGMS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 19050-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19050-1-AP (SGMS1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 19050-1-AP

  IP result of anti-SGMS1 (IP:19050-1-AP, 4ug; Detection:19050-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of human liver using 19050-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19050-1-AP (SGMS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 19050-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19050-1-AP (SGMS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-29, tissu cardiaque humain
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

19050-1-AP cible SGMS1 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SGMS1 Protéine recombinante Ag5246
• Nom complet: sphingomyelin synthase 1
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 25 kDa, 49 kDa
• Numéro d’acquisition GenBank: BC042899
• Symbole du gène: SGMS1
• Identification du gène (NCBI): 259230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sphingomyelin synthase (SMS), with two isoforms, SMS1 (SGMS1) and SMS2, is a key enzyme involved in the generation and development of SM. SMS can participate in inflammation, atherosclerosis, proliferation, apoptosis, differentiation and other functions(PMID:30535436). SMS1 is frequently downregulated in various solid cancers, more particularly in melanoma(PMID:31114500). SGMS1 has two isoforms.

Publications

Species	Application	Title
mouse,human	WB	Cancer Res; Aneuploid cell survival relies upon sphingolipid homeostasis.; Authors - Yun-Chi Tang ; KD Validated
human	IHC	J Clin Endocrinol Metab; Dysregulated sphingolipid metabolism in endometriosis.; Authors - Yie Hou Lee
mouse	WB,IHC	FASEB J; Sphingomyelin maintains the cutaneous barrier via regulation of the STAT3 pathway.; Authors - Mariko Komuro
mouse,human	WB,IF	Exp Neurol; Inhibition of sphingomyelin synthase 1 ameliorates alzheimer-like pathology in APP/PS1 transgenic mice through promoting lysosomal degradation of BACE1.; Authors - Mei-Hong Lu ; KD Validated
mouse	WB	iScience; The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.; Authors - Tirthankar Sinha
mouse	IF	Drug Des Devel Ther; Involvement of Huanglian Jiedu Decoction on Microglia with Abnormal Sphingolipid Metabolism in Alzheimer's Disease.; Authors - Yi-Yu Qi

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