Anticorps Polyclonal de lapin anti-SGOL1

• Phare
• Validé par KD/KO

SGOL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 16977-1-AP

Synonymes

hSgo1, NY BR 85, Serologically defined breast cancer antigen NY-BR-85, SGO, SGO1

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Galerie de données de validation

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  WB analysis using 16977-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16977-1-AP (SGOL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 16977-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 16977-1-AP (SGOL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 16977-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SGOL1 antibody (16977-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3, cellules PC-3, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

16977-1-AP cible SGOL1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SGOL1 Protéine recombinante Ag9700
• Nom complet: shugoshin-like 1 (S. pombe)
• Masse moléculaire calculée: 561aa,64 kDa; 292aa,34 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC017867
• Symbole du gène: SGOL1
• Identification du gène (NCBI): 151648
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SGOL1 (Shugoshin 1), a centromeric protein, plays an important role in mitosis (PMID: 23102702).

Publications

Species	Application	Title
human	IF	Cell Death Dis; Genome-wide CRISPR screen identifies BUB1 kinase as a druggable vulnerability in malignant pleural mesothelioma; Authors - Ece Cakiroglu
mouse	WB,IHC	Carcinogenesis; Tumor-promoting/progressing role of additional chromosome instability in hepatic carcinogenesis in Sgo1 (Shugoshin 1) haploinsufficient mice.; Authors - Hiroshi Y Yamada
human	IHC	Front Oncol; Deciphering the Role of Shugoshin-Like Protein 1 in Lung Adenocarcinoma: A Comprehensive Analysis and In Vitro Study.; Authors - Yixiao Yuan
mouse	IHC	Mol Carcinog; Antagonizing pathways leading to differential dynamics in colon carcinogenesis in Shugoshin1 (Sgo1)-haploinsufficient chromosome instability model.; Authors - Chinthalapally V Rao
human	WB,IHC,IF	BMC Med Genomics; Prognosis and biological function of SGOL1 in clear cell renal cell carcinoma: a multiomics analysis; Authors - Zezhong Yang ; KD Validated
human	WB	Gene; Integrative analysis of bulk RNA and single-cell sequencing data reveals a liquid-liquid phase separation signature for clear cell renal cell carcinoma; Authors - Hongbo Wang

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