Anticorps Polyclonal de lapin anti-SGTA

• Phare
• Validé par KD/KO

SGTA Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

20

Avis (3)

N° de cat : 11019-2-AP

Synonymes

Alpha SGT, alphaSGT, aSGT, hSGT, SGT, SGT1, SGTA, UBP, Vpu binding protein

---

Galerie de données de validation

• 

  WB analysis of K-562 using 11019-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11019-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11019-2-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11019-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11019-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11019-2-AP

  IP Result of anti-SGTA (IP:11019-2-AP, 4ug; Detection:11019-2-AP 1:1000) with HeLa cells lysate 2480ug.

• 

  IHC staining of human lung cancer using 11019-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11019-2-AP (SGTA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11019-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11019-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 11019-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11019-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11019-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11019-2-AP (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules HeLa, cellules SH-SY5Y, tissu de muscle squelettique humain, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de la prostate humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

11019-2-AP cible SGTA dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SGTA Protéine recombinante Ag1475
• Nom complet: small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 34-37 kDa
• Numéro d’acquisition GenBank: BC008885
• Symbole du gène: SGTA
• Identification du gène (NCBI): 6449
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.

Publications

Species	Application	Title
	WB	J Cell Biol; Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes.; Authors - Yu-Jie Chen
human	WB	PLoS Pathog; A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.; Authors - Christopher Paul Walczak ; KD Validated
	WB	PLoS Pathog; A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane.; Authors - Madhu Sudhan Ravindran
human	IHC	Int J Cancer; Knockdown of the co-chaperone SGTA results in the suppression of androgen and PI3K/AKT signaling and inhibition of prostate cancer cell proliferation.; Authors - Trotta Andrew P AP
mouse	WB	Int J Mol Sci; Isoquercitrin Attenuates Steatohepatitis by Inhibition of the Activated NLRP3 Inflammasome through HSP90; Authors - Ji Ma
mouse	IHC	Sci Rep; Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice.; Authors - Lisa K Philp ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

WEI (Verified Customer) (03-08-2022)

Specific band at predicted size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

WEI (Verified Customer) (11-12-2020)

A strong band at the predicted ~35kDa size.

• Applications: Western Blot
• Cell Tissue Type: Mouse hearts

FH

Tom (Verified Customer) (10-09-2020)

I saw a band at ~36kDa using this antibody. There are some non-specific bands present but the specific SGTA band was the strongest band.

• Applications: Western Blot