Anticorps Polyclonal de lapin anti-SH2D3C

SH2D3C Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC,ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 12882-1-AP

Synonymes

CHAT, Novel SH2 containing protein 3, NSP3, PRO34088, SH2 domain containing 3C, SH2D3C

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Galerie de données de validation

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  WB analysis of HEK-293 using 12882-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12882-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12882-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment using 12882-1-AP

  IP result of SH2D3C antibody (12882-1-AP for IP and Detection) with mouse brain tissue lysate.

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  IP experiment of mouse brain using 12882-1-AP

  IP Result of anti-SH2D3C (IP:12882-1-AP, 3ug; Detection:12882-1-AP 1:500) with mouse brain tissue lysate 6000ug.

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  IHC staining of human breast cancer using 12882-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12882-1-AP (SH2D3C antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu cérébral humain, tissu placentaire humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12882-1-AP cible SH2D3C dans les applications de WB, IP, IHC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SH2D3C Protéine recombinante Ag3887
• Nom complet: SH2 domain containing 3C
• Masse moléculaire calculée: 94 kDa, 86 kDa, 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC027962
• Symbole du gène: SH2D3C
• Identification du gène (NCBI): 10044
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SH2 domain-containing protein 3C (SH2D3C, or NSP3) gene encodes an adaptor protein and member of a cytoplasmic protein family involved in cell migration. The encoded protein was identified as an Eph receptor-binding protein which may be a positive regulator of TCR signaling (PMID: 10542222). SH2D3C is an adaptor protein that has been implicated in T cell activation and shown to associate with different components of the integrin signaling pathway ex vivo, moreover, its primary in vivo role of Sh2d3c in regulating B cell development and function has also been demonstrated (PMID: 20505138). SH2D3C has 6 isoforms with MW 94, 86, 77 and 55 kDa (refer to UniProt).

Publications

Species	Application	Title
rat	WB,IP	Neuroscience; Up-regulation of NSP3 by oligomeric Aβ accelerates neuronal death through Cas-independent Rap1A activation.; Authors - Fujiya Gomi