Anticorps Polyclonal de lapin anti-SH3BGRL2

• Phare
• Validé par KD/KO

SH3BGRL2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 21944-1-AP

Synonymes

FASH3, SH3BGRL2

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Galerie de données de validation

• 

  WB analysis of mouse brain using 21944-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 21944-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21944-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 21944-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 21944-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HEK-293 using 21944-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu hépatique de souris, tissu placentaire humain
• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral de souris, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

21944-1-AP cible SH3BGRL2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SH3BGRL2 Protéine recombinante Ag16638
• Nom complet: SH3 domain binding glutamic acid-rich protein like 2
• Masse moléculaire calculée: 107 aa, 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC040489
• Symbole du gène: SH3BGRL2
• Identification du gène (NCBI): 83699
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Am J Cancer Res; SH3BGRL2 exerts a dual function in breast cancer growth and metastasis and is regulated by TGF-β1.; Authors - Dou-Dou Li ; KD Validated
human	WB	Front Pharmacol; Astragalus Polysaccharides Inhibits Tumorigenesis and Lipid Metabolism Through miR-138-5p/SIRT1/SREBP1 Pathway in Prostate Cancer.; Authors - Shanqi Guo