Anticorps Polyclonal de lapin anti-Bif-1

• Phare
• Validé par KD/KO

Bif-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 15422-1-AP

Synonymes

SH3GLB1, Bax interacting factor 1, Bax-interacting factor 1, Bif1, Endophilin B1

---

Galerie de données de validation

• 

  WB analysis of mouse kidney using 15422-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15422-1-AP

  WB result of Bif-1 antibody (15422-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Bif-1 transfected HepG2 cells.

• 

  WB analysis of mouse heart using 15422-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 15422-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15422-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15422-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15422-1-AP (Bif-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15422-1-AP

  IP result of anti-Bif-1 (IP:15422-1-AP, 4ug; Detection:15422-1-AP 1:400) with HEK-293 cells lysate 2000 ug.

• 

  IHC staining of mouse kidney using 15422-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15422-1-AP (Bif-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 15422-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15422-1-AP (Bif-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15422-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Bif-1 antibody (15422-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, cellules HepG2, tissu cardiaque de souris, tissu placentaire de souris, tissu placentaire humain
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu rénal de souris, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

15422-1-AP cible Bif-1 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Bif-1 Protéine recombinante Ag7710
• Nom complet: SH3-domain GRB2-like endophilin B1
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC007455
• Symbole du gène: Bif-1
• Identification du gène (NCBI): 51100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SH3GLB1 also known as Bif-1, contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain, but shares no significant homology with members of the Bcl-2 family. SH3GLB1 is involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588).

Publications

Species	Application	Title
mouse	IF	Biomed Mater; Nano implant surface triggers autophagy through membrane curvature distortion to regulate the osteogenic differentiation; Authors - Guangwen Li
	WB	Mediators Inflamm; Sodium Houttuyfonate Ameliorates β-amyloid1-42-Induced Memory Impairment and Neuroinflammation through Inhibiting the NLRP3/GSDMD Pathway in Alzheimer's Disease.; Authors - Yuequan Zhao
rat	WB,IHC	Eur J Pharmacol; SUMO2-mediated SUMOylation of SH3GLB1 promotes ionizing radiation-induced hypertrophic cardiomyopathy through mitophagy activation.; Authors - Anbo Gao
human	WB	J Exp Clin Cancer Res; SH3GLB1-related autophagy mediates mitochondrial metabolism to acquire resistance against temozolomide in glioblastoma.; Authors - Chia-Hung Chien
mouse	WB	Oncotarget; Autophagy-related gene expression is an independent prognostic indicator of glioma.; Authors - Huixue Zhang
human	WB,ChIP	BMC Cancer; Role of SH3GLB1 in the regulation of CD133 expression in GBM cells; Authors - Chia-Hung Chien

{{ptg:RelatedPrimaryAntibodies}}