Anticorps Polyclonal de lapin anti-POSH

• Phare
• Validé par KD/KO

POSH Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 14649-1-AP

Synonymes

KIAA1494, Plenty of SH3s, POSH, Protein POSH, RING finger protein 142, RNF142, SH3 multiple domains protein 2, SH3MD2, SH3RF1

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Galerie de données de validation

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  WB analysis of mouse brain using 14649-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14649-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14649-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 14649-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14649-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14649-1-AP

  IP Result of anti-POSH (IP:14649-1-AP, 4ug; Detection:14649-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human gliomas using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules DU 145, cellules HEK-293, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Informations sur le produit

14649-1-AP cible POSH dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POSH Protéine recombinante Ag6243
• Nom complet: SH3 domain containing ring finger 1
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 93-100 kDa
• Numéro d’acquisition GenBank: BC053671
• Symbole du gène: SH3RF1
• Identification du gène (NCBI): 57630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POSH (plenty of SH3 domains) is a multidomain GTP-Rac1-interacting protein that acts as a scaffold for JNK activation, leading to apoptosis in neuronal and non-neuronal cells. It consists a RING-finger domain that possesses ubiquitin-ligase activity and four SH3 domains.

Publications

Species	Application	Title
drosophila	IF	J Cell Biol; Rab8, POSH, and TAK1 regulate synaptic growth in a Drosophila model of frontotemporal dementia.; Authors - Ryan J H West
mouse	WB	Int J Mol Sci; Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions.; Authors - Sunil Goodwani
mouse	IF	Hum Mol Genet; The Pro-Apoptotic JNK Scaffold POSH/SH3RF1 Mediates CHMP2BIntron5-Associated Toxicity in Animal Models of Frontotemporal Dementia.; Authors - Ryan J H West
human,mouse	WB,IF,IP	Expert Opin Ther Targets; POSH participates in epileptogenesis by increasing the surface expression of the NMDA receptor: a promising therapeutic target for epilepsy.; Authors - Xinshi Wang ; KD Validated
human,mouse	WB,IP	Cell Rep; POSH regulates assembly of the NMDAR/PSD-95/Shank complex and synaptic function.; Authors - Minghui Yao
human	WB	Int J Biol Macromol; Snakehead vesiculovirus hijacks SH3RF1 for replication via mediating K63-linked ubiquitination at K264 of the phosphoprotein; Authors - Xiangmou Qin