Anticorps Polyclonal de lapin anti-SHANK1

SHANK1 Polyclonal Antibody for WB, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 55059-1-AP

Synonymes

SHANK1, SPANK 1, SSTR interacting protein, SSTRIP, synamon

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Galerie de données de validation

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  WB analysis using 55059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55059-1-AP (SHANK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 55059-1-AP

  IP result of anti-SHANK1 (IP:55059-1-AP, 4ug; Detection:55059-1-AP 1:400) with HEK-293T cells lysate 1155 ug.

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  IF Staining of mouse eye using 55059-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SHANK1 antibody (55059-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse eye using 55059-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SHANK1 antibody (55059-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules Neuro-2a
• Résultats positifs en IP: cellules HEK-293T,
• Résultats positifs en IF-P: tissu oculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

55059-1-AP cible SHANK1 dans les applications de WB, IHC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SH3 and multiple ankyrin repeat domains 1
• Masse moléculaire calculée: 225 kDa
• Poids moléculaire observé: 159-225 kDa
• Numéro d’acquisition GenBank: NM_016148
• Symbole du gène: SHANK1
• Identification du gène (NCBI): 50944
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SHANK1 belongs to the SHANK family. It seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. SHANK1 may play a role in the structural and functional organization of the dendritic spine and synaptic junction. The antibody is specific to SHANK1.

Publications

Species	Application	Title
mouse	WB	Cell Res; METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation.; Authors - Zeyu Zhang
rat	WB	Neural Regen Res; Protective effects of Bushen Tiansui decoction on hippocampal synapses in a rat model of Alzheimer's disease.; Authors - Shan Hui
human,mouse	WB,IP,IHC,CoIP	J Biochem Mol Toxicol; USP18 Confers Paclitaxel Resistance in Non-Small Cell Lung Cancer by Stabilizing SHANK1 Expression Via Deubiquitination; Authors - Lixun Chai

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