Anticorps Polyclonal de lapin anti-SHARPIN

• Phare
• Validé par KD/KO

SHARPIN Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

88

Avis (1)

N° de cat : 14626-1-AP

Synonymes

hSIPL1, SHARPIN, SIPL1

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Galerie de données de validation

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  WB analysis using 14626-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14626-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14626-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14626-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14626-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14626-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14626-1-AP (SHARPIN Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 14626-1-AP

  IP Result of anti-SHARPIN (IP:14626-1-AP, 3ug; Detection:14626-1-AP 1:1500) with Raji cells lysate 2520ug.

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  IHC staining of human lymphoma using 14626-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 14626-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 14626-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 14626-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14626-1-AP (SHARPIN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of Jurkat using 14626-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SHARPIN antibody (14626-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules Jurkat, cellules Raji, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu de lymphome humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 82 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 12 publications below
• CoIP: See 2 publications below

Informations sur le produit

14626-1-AP cible SHARPIN dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SHARPIN Protéine recombinante Ag6201
• Nom complet: SHANK-associated RH domain interactor
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC025244
• Symbole du gène: SHARPIN
• Identification du gène (NCBI): 81858
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SHARPIN is as a third component of the linear ubiquitin chain assembly complex (LUBAC), recruited to the CD40 and TNF receptor signaling complexes together with its other constituents, HOIL1 and HOIP(PMID:21455173).SHARPIN is enriched in the postsynaptic density and forms a complex with SHANK in heterologous cells and brain(PMID:11178875).It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Nature; OTULIN limits cell death and inflammation by deubiquitinating LUBAC.; Authors - Klaus Heger
mouse	WB,IP	Nature; LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis.; Authors - Nieves Peltzer
human	WB,IP	Cell; OTULIN Antagonizes LUBAC Signaling by Specifically Hydrolyzing Met1-Linked Polyubiquitin.; Authors - Keusekotten Kirstin K
human	WB	Cell; The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.; Authors - Rune Busk Damgaard
mouse	WB	Cancer Cell; A Dual Role of Caspase-8 in Triggering and Sensing Proliferation-Associated DNA Damage, a Key Determinant of Liver Cancer Development.; Authors - Yannick Boege
mouse	WB	Nat Immunol; Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis.; Authors - Yang Shuo S

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katharina (Verified Customer) (07-17-2019)

The antibody worked as expected, detecting a nice band at the right size. Recommended

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: A549 cells /immunoprecipitates

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