Anticorps Recombinant de lapin anti-SHBG

SHBG Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230356F10

N° de cat : 83096-1-RR

Synonymes

Testis-specific androgen-binding protein, Sex steroid-binding protein, Sex steroid binding protein, Sex hormone-binding globulin, SBP

---

Galerie de données de validation

• 

  WB analysis of human placenta using 83096-1-RR

  human placenta tissue were subjected to SDS PAGE followed by western blot with 83096-1-RR (SHBG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 83096-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SHBG Recombinant antibody (83096-1-RR, Clone:230356F10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83096-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83096-1-RR against Human SHBG were performed. The affinity constant is 0.38 nM.

---

Applications testées

• Résultats positifs en WB: tissu placentaire humain,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83096-1-RR cible SHBG dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: sex hormone-binding globulin
• Masse moléculaire calculée: 402 aa, 44 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC101785
• Symbole du gène: SHBG
• Identification du gène (NCBI): 6462
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.

{{ptg:RelatedPrimaryAntibodies}}