Anticorps Polyclonal de lapin anti-SHC

• Phare
• Validé par KD/KO

SHC Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 10054-1-AP

Synonymes

SH2 domain protein C1, SHC, SHC transforming protein 1, SHC transforming protein 3, SHC transforming protein A, SHC1, SHCA

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Galerie de données de validation

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  WB analysis using 10054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 10054-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10054-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10054-1-AP (SHC antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10054-1-AP (SHC antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10054-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10054-1-AP (SHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules C6, cellules A431, cellules HeLa, cellules HUVEC, cellules U2OS, tissu cutané de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 3 publications below

Informations sur le produit

10054-1-AP cible SHC dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SHC Protéine recombinante Ag0103
• Nom complet: SHC (Src homology 2 domain containing) transforming protein 1
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: NM_183001
• Symbole du gène: SHC1
• Identification du gène (NCBI): 6464
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

Publications

Species	Application	Title
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu
mouse	IF	Oxid Med Cell Longev; Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury.; Authors - Rong Fu ; KD Validated
mouse	WB	Aging (Albany NY); RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.; Authors - Chan Chen
mouse	WB	Aging (Albany NY); Targeting of miR-96-5p by catalpol ameliorates oxidative stress and hepatic steatosis in LDLr-/- mice via p66shc/cytochrome C cascade.; Authors - Yukun Zhang
rat	WB	J Ethnopharmacol; Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via MK/integrin signaling pathway; Authors - Chuankui Fu
mouse	WB	Stem Cell Res Ther; Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.; Authors - Jie Zhou