Anticorps Polyclonal de lapin anti-SHMT1

• Phare
• Validé par KD/KO

SHMT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (3)

N° de cat : 30192-1-AP

Synonymes

EC:2.1.2.1, CSHMT

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Galerie de données de validation

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  WB analysis using 30192-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30192-1-AP (SHMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 30192-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 30192-1-AP (SHMT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 30192-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHMT1 antibody (30192-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 30192-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SHMT1 (30192-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MCF-7, cellules MOLT-4, cellules T-47D, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

30192-1-AP cible SHMT1 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SHMT1 Protéine recombinante Ag31269
• Nom complet: serine hydroxymethyltransferase 1 (soluble)
• Masse moléculaire calculée: 483 aa, 53 kDa
• Poids moléculaire observé: 45-53 kDa
• Numéro d’acquisition GenBank: BC038598
• Symbole du gène: SHMT1
• Identification du gène (NCBI): 6470
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SHMT1 is the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. This antibody can detect both 49 kDa and 53 kDa isoforms in western blotting.

Publications

Species	Application	Title
human	WB,IHC	Sci Rep; Integrative bioinformatics approach identifies novel drug targets for hyperaldosteronism, with a focus on SHMT1 as a promising therapeutic candidate; Authors - Minyue Jia
human,mouse	WB	Biol Trace Elem Res; Activation of the De Novo Serine Synthesis Pathway and Disruption of Insulin Signaling Induced by Supplemental SeMet in Vitro; Authors - Shuo Zhan
human	WB	Adv Sci (Weinh); Phosphorylated SHMT2 Regulates Oncogenesis Through m6 A Modification in Lung Adenocarcinoma; Authors - Tianyu Han ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Quentin (Verified Customer) (07-23-2025)

Worked nice on mouse brain tissues

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1/500
• Cell Tissue Type: mouse brain tissue

FH

Hua (Verified Customer) (04-26-2024)

Very good antibody for WB. Strong signal and clean background. It is a better antibody than 14149-1-AP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: Mouse live and mouse brain

SHMT1 Antibody Western Blot validation (1:8000 dilution) in Mouse live and mouse brain (Cat no:30192-1-AP)

FH

Adrian (Verified Customer) (03-22-2024)

Very good customer help, quick delivery and good quality antibodies

• Applications: Western Blot

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