Anticorps Polyclonal de lapin anti-SHOC2/Sur-8

• Phare
• Validé par KD/KO

SHOC2/Sur-8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 17561-1-AP

Synonymes

SHOC2, Sur-8, KIAA0862, Leucine-rich repeat protein SHOC-2, Protein soc 2 homolog

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Galerie de données de validation

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  WB analysis using 17561-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17561-1-AP (Sur-8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17561-1-AP

  WB result of SHOC2/Sur-8 antibody (17561-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHOC2/Sur-8 transfected MCF-7 cells.

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  WB analysis of Jurkat using 17561-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17561-1-AP (Sur-8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17561-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17561-1-AP (Sur-8 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 17561-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17561-1-AP (Sur-8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17561-1-AP (Sur-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 17561-1-AP (Sur-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17561-1-AP (Sur-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17561-1-AP (Sur-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17561-1-AP (Sur-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17561-1-AP (Sur-8 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 17561-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17561-1-AP (Sur-8 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 17561-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Sur-8 antibody (17561-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 17561-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Sur-8 antibody (17561-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules Jurkat, cellules U-251, tissu cérébral de souris, tissu testiculaire de rat
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu cérébral de souris, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IP: See 4 publications below

Informations sur le produit

17561-1-AP cible SHOC2/Sur-8 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SHOC2/Sur-8 Protéine recombinante Ag11754
• Nom complet: soc-2 suppressor of clear homolog (C. elegans)
• Masse moléculaire calculée: 582 aa, 65 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC050445
• Symbole du gène: SHOC2/Sur-8
• Identification du gène (NCBI): 8036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SHOC2 is also named as Sur-8. The SHOC2 protein, along with MRAS and PP1C, forms a high affinity, heterotrimeric holoenzyme that activates RAF kinases by dephosphorylating a specific phosphoserine (PMID: 37074066). SHOC2, a key protein mediating the Ras-Raf-ERK pathway, could act as a scaffolding protein to facilitate the activation of the pathway by mediating the interaction of key components of the pathway (PMID: 37170083). The scaffold protein Shoc2 is a critical modulator of ERK1/2 signals, and mutations in the shoc2 gene lead to the human developmental disease known as Noonan-like syndrome with loose anagen hair (NSLH) (PMID: 36265687).

Publications

Species	Application	Title
human	WB,IP	J Clin Invest; Desmoglein-1/Erbin interaction suppresses ERK activation to support epidermal differentiation.; Authors - Harmon Robert M RM
mouse	WB	EMBO J; Phosphorylation of Pkp1 by RIPK4 regulates epidermal differentiation and skin tumorigenesis.; Authors - Philbert Lee
monkey	WB,IP	J Cell Sci; Spatial control of Shoc2 scaffold-mediated ERK1/2 signaling requires remodeling activity of the ATPase PSMC5.; Authors - Eun Ryoung Jang
human	WB,IP	Mol Cell Biol; HUWE1 Is a Molecular Link Controlling RAF-1 Activity Supported by the Shoc2 Scaffold.; Authors - Eun Ryoung Jang
human	IHC	Mol Cancer Res; SHOC2 Is a Critical Modulator of Sensitivity to EGFR-TKIs in Non-Small Cell Lung Cancer Cells.; Authors - Hideki Terai ; KO Validated
human	WB,IP	J Cell Sci; The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.; Authors - Patricia Wilson ; KD Validated

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