Anticorps Polyclonal de lapin anti-Siglec-7/CD328

Siglec-7/CD328 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 13939-1-AP

Synonymes

SIGLEC7, Siglec-7, Sialic acid-binding Ig-like lectin 7, D siglec, CD328

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Galerie de données de validation

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  WB analysis using 13939-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 13939-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13939-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13939-1-AP (Siglec-7/CD328 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13939-1-AP (Siglec-7/CD328 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13939-1-AP (Siglec-7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13939-1-AP (Siglec-7 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules U-937, tissu hépatique humain, tissu placentaire de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu hépatique humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

13939-1-AP cible Siglec-7/CD328 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Siglec-7/CD328 Protéine recombinante Ag4220
• Nom complet: sialic acid binding Ig-like lectin 7
• Masse moléculaire calculée: 467 aa, 51 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC028150
• Symbole du gène: Siglec-7
• Identification du gène (NCBI): 27036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).

Publications

Species	Application	Title
human	WB,IHC,IF	Oncol Lett; Siglec-7 is a predictive biomarker for the efficacy of cancer vaccination against metastatic colorectal cancer.; Authors - Kensuke Yamada
		FEBS Open Bio; TWIST1 induces phenotypic switching of vascular smooth muscle cells by downregulating p68 and microRNA-143/145.; Authors - Jing Zhang
human	WB,IF	Commun Biol; ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3Gal1) synthesis of Siglec ligands mediates anti-tumour immunity in prostate cancer; Authors - Rebecca Garnham

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

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