Anticorps Polyclonal de lapin anti-Siglec-7

Siglec-7 Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (1)

N° de cat : 13939-1-AP

Synonymes

AIRM 1, AIRM1, CD328, CDw328, D siglec, p75, p75/AIRM1, QA79, QA79 membrane protein, SIGLEC 7, SIGLEC7, Siglec-7

---

Galerie de données de validation

• 

  WB analysis of U-937 using 13939-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13939-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 13939-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 13939-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13939-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13939-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13939-1-AP (Siglec-7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  IHC staining of human tonsillitis using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 13939-1-AP (Siglec-7 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13939-1-AP (Siglec-7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 13939-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 13939-1-AP (Siglec-7 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of U-937 using 13939-1-AP

  1X10^6 U-937 cells were stained with 0.2ug Siglec-7 antibody (13939-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: cellules U-937, cellules A549, cellules K-562, cellules L02, tissu hépatique humain, tissu placentaire humain
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu hépatique humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules U-937

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13939-1-AP cible Siglec-7 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Siglec-7 Protéine recombinante Ag4220
• Nom complet: sialic acid binding Ig-like lectin 7
• Masse moléculaire calculée: 467 aa, 51 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC028150
• Symbole du gène: SIGLEC7
• Identification du gène (NCBI): 27036
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sialic acid binding Ig-like lectin 7 (Siglec-7), also known as CD328 or p75/AIRM-1, is a member of the Siglec family of glycan-recognition proteins. Siglec-7 is a 75-kDa type-I transmembrane protein consisting of three extracellular immunoglobulin-like domains that comprise an N-terminal V-set domain and two C2-set domains, a transmembrane region and a cytoplasmic tail containing two tyrosine residues embodied in immunoreceptor tyrosine-based inhibition motif-like motifs (PMID: 32322597; 10567377). It is mainly expressed on immune cells, with low levels on granulocytes, intermediate levels on monocytes, and relatively high levels on a major subset of natural killer cells and a minor subset of CD8+ T cells (PMID: 10567377). Siglec-7 is an inhibitory receptor that negatively regulates the function of NK cells and modulates the immune response through the interaction of sialic acid-containing ligands (PMID: 27312286).

Publications

Species	Application	Title
human	WB,IHC,IF	Oncol Lett; Siglec-7 is a predictive biomarker for the efficacy of cancer vaccination against metastatic colorectal cancer.; Authors - Kensuke Yamada

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue. Antigen retrieval Tris-EDTA pH 9.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate