Anticorps Polyclonal de lapin anti-SIGMAR1

• Phare
• Validé par KD/KO

SIGMAR1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

35

N° de cat : 15168-1-AP

Synonymes

hSigmaR1, OPRS1, SIG 1R, Sigma 1 type opioid receptor, Sigma1 receptor, Sigma1R, SIGMAR1, SR BP, SR BP1, SR31747 binding protein, SRBP

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Galerie de données de validation

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  WB analysis using 15168-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15168-1-AP (SIGMAR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15168-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15168-1-AP (SIGMAR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of rat brain using 15168-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 15168-1-AP (SIGMAR1 antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, tissu de côlon de rat, tissu de côlon de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 28 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• CoIP: See 2 publications below

Informations sur le produit

15168-1-AP cible SIGMAR1 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIGMAR1 Protéine recombinante Ag7351
• Nom complet: sigma non-opioid intracellular receptor 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC004899
• Symbole du gène: SIGMAR1
• Identification du gène (NCBI): 10280
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIGMAR1, also named as OPRS1, SRBP, SIG-1R and AAG8, belongs to the ERG2 family. It is an endoplasmic reticulum chaperone that binds a wide variety of ligands, including neurosteroids, psychostimulants, and dextrobenzomorphans. SIGMAR1 is ubiquitously expressed, and is enriched in motor neurons of the brain and spinal cord. It plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. SIGMAR1 plays a role in several other cell functions including proliferation, survival and death. SDS-PAGE and Western blot analysis showed that SIGMAR1 has an apparent molecular mass of 28 kDa. SIGMAR1 has 5 isoforms with MW 21-27kDa and 12kDa.

Publications

Species	Application	Title
mouse	WB	Nat Commun; ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.; Authors - Yuanyuan Zhao
human	WB	Ann Neurol; Hereditary Spastic Paraplegia-Linked REEP1 Modulates ER-Mitochondria Contacts.; Authors - Youngshin Lim
		Proc Natl Acad Sci U S A; DJ-1 regulates the integrity and function of ER-mitochondria association through interaction with IP3R3-Grp75-VDAC1.; Authors - Yi Liu
mouse	WB	Schizophr Bull; Allosteric Modulation of the Sigma-1 Receptor Elicits Antipsychotic-like Effects.; Authors - Jiali Chen
mouse	WB	Cell Mol Life Sci; PACS2-TRPV1 axis is required for ER-mitochondrial tethering during ER stress and lung fibrosis.; Authors - Jessica Knoell
zebrafish	WB	Int J Mol Sci; Sigma-1 Receptor Is Critical for Mitochondrial Activity and Unfolded Protein Response in Larval Zebrafish.; Authors - Lucie Crouzier ; KD Validated

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