Anticorps Polyclonal de lapin anti-SIN3A

SIN3A Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 14638-1-AP

Synonymes

KIAA0700

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Galerie de données de validation

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  WB analysis of Jurkat using 14638-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14638-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14638-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14638-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14638-1-AP (SIN3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 14638-1-AP

  IP result of anti-SIN3A (IP:14638-1-AP, 4ug; Detection:14638-1-AP 1:500) with Jurkat cells lysate 3600ug.

• 

  IF Staining of HepG2 using 14638-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SIN3A antibody (14638-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, cellules K-562
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14638-1-AP cible SIN3A dans les applications de WB, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIN3A Protéine recombinante Ag6219
• Nom complet: SIN3 homolog A, transcription regulator (yeast)
• Masse moléculaire calculée: 145 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC066364
• Symbole du gène: SIN3A
• Identification du gène (NCBI): 25942
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Theranostics; SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.; Authors - Ti-Chun Chan
human	WB,CoIP	Int J Biol Sci; TET1s deficiency exacerbates oscillatory shear flow-induced atherosclerosis.; Authors - Kai Qu
		Cell Rep; ZNF451 favors triple-negative breast cancer progression by enhancing SLUG-mediated CCL5 transcriptional expression; Authors - Yu Zhang
mouse	WB,IP	Nat Commun; Glucocorticoids increase adiposity by stimulating Krüppel-like factor 9 expression in macrophages; Authors - Yinliang Zhang
rat	WB	Bioact Mater; Chitosan/PLGA-based tissue engineered nerve grafts with SKP-SC-EVs enhance sciatic nerve regeneration in dogs through miR-30b-5p-mediated regulation of axon growth; Authors - Miaomei Yu

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