Anticorps Polyclonal de lapin anti-SIPA1

SIPA1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 26793-1-AP

Synonymes

GTPase-activating protein Spa-1, p130 SPA 1, p130 SPA-1, Signal-induced proliferation-associated protein 1, Sipa 1

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Galerie de données de validation

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  WB analysis of Raji using 26793-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 26793-1-AP (SIPA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 26793-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26793-1-AP (SIPA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 26793-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26793-1-AP (SIPA1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HEK-293T
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

26793-1-AP cible SIPA1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIPA1 Protéine recombinante Ag25383
• Nom complet: signal-induced proliferation-associated 1
• Masse moléculaire calculée: 1042 aa, 112 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC010492
• Symbole du gène: SIPA1
• Identification du gène (NCBI): 6494
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIPA1 (Signal-induced proliferation-associated protein 1) is also named as SPA1, p130 SPA-1 and GTPase-activating protein Spa-1. SIPA1, a GTPase activating protein, was discovered in proliferating lymphocytes as mitogen-induced nuclear protein. SIPA1 promotes catalyzation of hydrolyze Rap1GTP/GDP and is known to be a negative regulator of Ras-related protein, which transduces the signals for various cellular functions, including development, cellular proliferation, and cellular adhesion (PMID:28237246). Transcription of fibronectin 1, which is crucial for cell junction and migration of triple-negative breast cancer (TNBC) cells, was regulated by SIPA1 in a DBR-dependent manner. SIPA1 was highly expressed in metastatic TNBC. SIPA1 served as a TF, promoting TNBC migration, invasion, and metastasis (PMID: 37500797).

Publications

Species	Application	Title
human	WB,IHC	Transl Oncol; Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.; Authors - Xinlu Liu

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