Anticorps Polyclonal de lapin anti-SIRP Beta 1

SIRP Beta 1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 11811-1-AP

Synonymes

CD172b, SIRP BETA 1, SIRPB1

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Galerie de données de validation

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  WB analysis using 11811-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 11811-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11811-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11811-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules U-937, cellules A375, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

11811-1-AP cible SIRP Beta 1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIRP Beta 1 Protéine recombinante Ag2478
• Nom complet: signal-regulatory protein beta 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC025286
• Symbole du gène: SIRPB1
• Identification du gène (NCBI): 10326
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIRP Beta 1 is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP Beta 1 interacts with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. SIRP Beta 1 also participates in the recruitment of tyrosine kinase SYK.

Publications

Species	Application	Title
human	IHC	PLoS One; SNPs array karyotyping reveals a novel recurrent 20p13 amplification in primary myelofibrosis.; Authors - Visani Giuseppe G
human	IHC	Neuropathology; Immunohistochemical characterization of microglia in Nasu-Hakola disease brains.; Authors - Satoh Jun-Ichi J