Anticorps Polyclonal de lapin anti-SIX1

• Phare
• Validé par KD/KO

SIX1 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 10709-1-AP

Synonymes

BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39

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Galerie de données de validation

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  WB analysis of A2780 using 10709-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 10709-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10709-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10709-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10709-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10709-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10709-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10709-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10709-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10709-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10709-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10709-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10709-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10709-1-AP

  IP Result of anti-SIX1 (IP:10709-1-AP, 3ug; Detection:10709-1-AP 1:1000) with HEK-293 cells lysate 2560ug.

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  IF Staining of epithelial tumor cells using 10709-1-AP

  IF result of anti-SIX1 (1:50) in p53-/- mammary epithelial tumor cells. Blue: hoechst nuclear stain, Red: SIX1 stain the mesenchymal cell which have undergone EMT.

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules HEK-293, cellules HepG2, cellules L02, cellules PC-3, cellules SKOV-3
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IF: cellules tumorales épithéliales

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 15 publications below
• IHC: See 8 publications below
• IF: See 8 publications below

Informations sur le produit

10709-1-AP cible SIX1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIX1 Protéine recombinante Ag1044
• Nom complet: SIX homeobox 1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32-35 kDa
• Numéro d’acquisition GenBank: BC008874
• Symbole du gène: SIX1
• Identification du gène (NCBI): 6495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.

Publications

Species	Application	Title
human	WB,IHC	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li ; KO Validated
mouse	WB	Nat Commun; Muscle progenitor specification and myogenic differentiation are associated with changes in chromatin topology.; Authors - Nan Zhang
mouse		PLoS Biol; Dynamic enhancers control skeletal muscle identity and reprogramming.; Authors - Krithika Ramachandran
human	WB	Theranostics; O-GlcNAcylation of SIX1 enhances its stability and promotes Hepatocellular Carcinoma Proliferation.; Authors - Yi Chu ; KD Validated
human	IF	Oncogene; The homeoprotein SIX1 controls cellular senescence through the regulation of p16INK4A and differentiation-related genes.; Authors - I Adrados
human	WB	Mol Ther Oncolytics; miR-489-3p/SIX1 Axis Regulates Melanoma Proliferation and Glycolytic Potential.; Authors - Xuhui Yang ; KD Validated