Anticorps Polyclonal de lapin anti-SKIL

• Phare
• Validé par KD/KO

SKIL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 19218-1-AP

Synonymes

SKI like oncogene, Ski like protein, Ski related oncogene, Ski related protein, SKIL, SNO, SnoA, SnoI, SnoN

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Galerie de données de validation

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  WB analysis using 19218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 19218-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19218-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19218-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19218-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19218-1-AP (SKIL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 19218-1-AP

  IP Result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug.

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  IP experiment of HepG2 using 19218-1-AP

  IP Result of anti-SKIL (IP:19218-1-AP, 4ug; Detection:19218-1-AP 1:500) with HepG2 cells lysate 1600ug.

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  IHC staining of human endometrial cancer using 19218-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 19218-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 19218-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 19218-1-AP (SKIL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 19218-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 19218-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKIL antibody (19218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HepG2, cellules MCF-7, tissu de muscle squelettique humain
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

19218-1-AP cible SKIL dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SKIL Protéine recombinante Ag5282
• Nom complet: SKI-like oncogene
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: BC059386
• Symbole du gène: SKIL
• Identification du gène (NCBI): 6498
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SKIL, also known as SnoN, is a mediator of the transforming growth factor-β (TGF-β) signaling pathway and exerts pro-oncogenic activity through inhibiting TGF-β/Smad pathway(PMID: 19114989). SKIL is involved in reduction of autophagy and subsequent tumor cell apoptosis in arsenic trioxide-treated promyelocytic leukemia and ovarian carcinoma. In addition, SKIL may regulate cell division or differentiation in response to extracellular signals.

Publications

Species	Application	Title
human	CoIP	Cell Death Differ; PIAS1 and TIF1γ collaborate to promote SnoN SUMOylation and suppression of epithelial-mesenchymal transition.; Authors - Ayan Chanda
mouse	WB	J Med Chem; Structure-Guided Design of a Small-Molecule Activator of Sirtuin-3 that Modulates Autophagy in Triple Negative Breast Cancer.; Authors - Jin Zhang
		J Neurosci; The Transcriptional Regulator SnoN Promotes the Proliferation of Cerebellar Granule Neuron Precursors in the Postnatal Mouse Brain.; Authors - Xiaoying Chen
human	WB	Mol Cell Proteomics; Quantitative ubiquitylome analysis reveals specificity of RNF111/Arkadia E3 ubiquitin ligase for its degradative substrates SKI and SKIL/SnoN in TGF-β signaling pathway; Authors - Victor Laigle
human	WB,IHC,IF	Apoptosis; SKI knockdown suppresses apoptosis and extracellular matrix degradation of nucleus pulposus cells via inhibition of the Wnt/β-catenin pathway and ameliorates disc degeneration.; Authors - Zuo-Long Wu ; KD Validated
human	WB	J Cell Mol Med; MDM2 is implicated in high-glucose-induced podocyte mitotic catastrophe via Notch1 signalling.; Authors - Hui Tang