Anticorps Monoclonal anti-SLAM/CD150

SLAM/CD150 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

mouse / IgM

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

5B4G1

N° de cat : 60043-1-Ig

Synonymes

CD150, CDw150, IPO 3, SLAM, SLAMF1

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Galerie de données de validation

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  WB analysis of Raji using 60043-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60043-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 60043-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HUVEC, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

60043-1-Ig cible SLAM/CD150 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: singe
• Hôte / Isotype: mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLAM/CD150 Protéine recombinante Ag1386
• Nom complet: signaling lymphocytic activation molecule family member 1
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC012602
• Symbole du gène: SLAMF1
• Identification du gène (NCBI): 6504
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation de l'acide caprylique/du sulfate d'ammonium
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.

Publications

Species	Application	Title
monkey	WB	Animals (Basel); Signaling Lymphocytic Activation Molecule Family Member 1 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication; Authors - Haofei Song ; KD Validated