Anticorps Polyclonal de lapin anti-SLC12A1/NKCC2

• Phare
• Validé par KD/KO

SLC12A1/NKCC2 Polyclonal Antibody for WB, IHC, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(32)

N° de cat : 18970-1-AP

Synonymes

NKCC2, SLC12A1, BSC1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 18970-1-AP

  WB result of NKCC2 antibody (18970-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKCC2 transfected HEK-293 cells.

• 

  WB analysis using 18970-1-AP

  Boiled or non-boiled lysates of mouse kidney were subjected to SDS PAGE followed by western blot with 18970-1-AP (NKCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18970-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse Embryonic kidney tissue using 18970-1-AP

  IF rsult of SLC12A1 antibody (18970-1-AP, 1:900) with mouse Embryonic kidneys from E16.5 (fixed with 4% PFA at 4C for 30 min, cryosections 5% goat serum/PBST was used for blocking and antibody incubation). Red: anti-rabbit Alexa555, Blue: Hoechst.

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu rénal de souris incubé à 37 °C
• Résultats positifs en IHC: tissu rénal humain, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu rénal embryonnaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 7 publications below
• IF: See 22 publications below

Informations sur le produit

18970-1-AP cible SLC12A1/NKCC2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC12A1/NKCC2 Protéine recombinante Ag5325
• Nom complet: solute carrier family 12 (sodium/potassium/chloride transporters), member 1
• Masse moléculaire calculée: 121 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC040138
• Symbole du gène: SLC12A1/NKCC2
• Identification du gène (NCBI): 6557
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human		Cell Stem Cell; Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk.; Authors - Sara E Howden
	IF	Nat Protoc; Generation of proximal tubule-enhanced kidney organoids from human pluripotent stem cells; Authors - Jessica M Vanslambrouck
human	IF	Nat Commun; Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids; Authors - Jessica M. Vanslambrouck
human	IF	J Am Soc Nephrol; A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids.; Authors - Jessica M Vanslambrouck
mouse	IF	J Am Soc Nephrol; Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney.; Authors - Sierra S Marable
mouse,human	WB,IHC	Mol Ther; Tubular-specific CDK12 knockout causes a defect in urine concentration due to premature cleavage of the slc12a1 gene.; Authors - Bin Wang

{{ptg:RelatedPrimaryAntibodies}}