Anticorps Polyclonal de lapin anti-SLC13A3

SLC13A3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 26182-1-AP

Synonymes

NADC3, Sodium-dependent high-affinity dicarboxylate transporter 2, SDCT2, NaDC-3, NaC3

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Galerie de données de validation

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  WB analysis of mouse kidney using 26182-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26182-1-AP (SLC13A3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26182-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26182-1-AP (SLC13A3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 26182-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26182-1-AP (SLC13A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 26182-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26182-1-AP (SLC13A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293
• Résultats positifs en IHC: tissu rénal de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

26182-1-AP cible SLC13A3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC13A3 Protéine recombinante Ag24183
• Nom complet: solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 3
• Masse moléculaire calculée: 602 aa, 67 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC035966
• Symbole du gène: SLC13A3
• Identification du gène (NCBI): 64849
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC13A3, also named NADC3 and SDCT2, belongs to the SLC13A/DASS transporter (TC 2.A.47) family and NADC subfamily. SLC13A3 encodes the plasma membrane Na+ /Dicarboxylate Cotransporter 3 (NaDC3), which imports inside the cell four to six carbon dicarboxylates as well as N-acetylaspartate (NAA). SLC13A3 is mainly expressed in kidney, but also in brain, liver, placenta and eye (PMID: 30635937). This antibody detected the 55-60 kDa band in SDS-PAGE. However, the 55 kDa (nonglycosylated) and 85 kDa (glycosylated) bands have been reported in the article (PMID: 27053689).

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Succinate Accumulation Is Associated with a Shift of Mitochondrial Respiratory Control and HIF-1α Upregulation in PTEN Negative Prostate Cancer Cells.; Authors - Anja Weber
human	IHC	Cell Metab; Adipocyte-derived glutathione promotes obesity-related breast cancer by regulating the SCARB2-ARF1-mTORC1 complex; Authors - Chenxi Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joo-Seop (Verified Customer) (07-23-2025)

It marks a subset of proximal tubules in the mouse kidney at P9.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse kidney

SLC13A3 Antibody Immunofluorescence validation (1:100 dilution) in mouse kidney (Cat no:26182-1-AP)

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